Enhancement of permeability of glycerol with ultrasound in human normal and cancer breast tissues in vitro using optical coherence tomography

Here we examined the effect of human normal breast (NB) and breast cancer (BC) tissues in vitro after treatment with glycerol in conjunction with ultrasound (surgeonperformed, SP) by OCT for functional imaging to monitor. 60% glycerol (G) and SP was simultaneously applied for 5 min. Depth- and time-resolved profiles for OCT signal enhancement were presented. The results show that OCT imaging depth of breast tissues after treatment with 60% G in combined with SP more obviously improved than that after application of glycerol alone. The permeability coefficient of 60% glycerol in 60% G/NB, 60% G/BC, 60% G/SP/NB, and 60% G/SP/BC were (0.91±0.02)×10-5 cm/s, (3.22±0.09)×10-5 cm/s, (1.63±0.04)×10-5 cm/s, and (7.98±0.19)×10-5 cm/s, respectively. The permeability coefficient of glycerol in 60% G/SP/NB was 1.84-fold than that in 60% G/NB, but there was 2.54-fold on permeability coefficient comparing 60% G/SP/BC with that in 60% G/BC. The results from this study indicated that the permeability coefficient of glycerol not only in normal breast tissues, but also in breast cancer tissues after treatment with ultrasound is larger than that in tissues without ultrasound.

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