Synthesis and antibacterial activity of silver nanoparticles with different sizes

Silver nanoparticles with different sizes (7, 29, and 89 nm mean values) were synthesized using gallic acid in an aqueous chemical reduction method. The nanoparticles were characterized using transmission electron microscopy (TEM), dynamic light scattering (DLS), X-ray diffraction (XRD), and ultraviolet–visible (UV–Vis) absorption spectroscopy; the antibacterial activity was assessed using the standard microdilution method, determining the minimum inhibitory concentration (MIC) according to the National Committee for Clinical Laboratory Standards. From the microscopies studies (TEM) we observed that silver nanoparticles have spherical (7 and 29 nm) and pseudospherical shape (89 nm) with a narrow size distribution. The sizes of the silver nanoparticles were controlled by varying some experimental conditions. It was found that the antibacterial activity of the nanoparticles varies when their size diminishes.

[1]  J. Szpunar.  ICOTOM-12 : proceedings of the twelfth International Conference on Textures of Materials, McGill University, Montreal, Canada, August 9-13, 1999 , 1999 .

[2]  K. W. Kwong,et al.  Ambient Light Reduction Strategy to Synthesize Silver Nanoparticles and Silver-Coated TiO2 with Enhanced Photocatalytic and Bactericidal Activities , 2003 .

[3]  Milan Kolar,et al.  Silver colloid nanoparticles: synthesis, characterization, and their antibacterial activity. , 2006, The journal of physical chemistry. B.

[4]  C. Ni,et al.  Antibacterial properties of silver-doped titania. , 2007, Small.

[5]  Dae Hong Jeong,et al.  Antimicrobial effects of silver nanoparticles. , 2007, Nanomedicine : nanotechnology, biology, and medicine.

[6]  N. Jana,et al.  Growing Small Silver Particle as Redox Catalyst , 1999 .

[7]  A. Bard,et al.  Interaction of silver(I) ions with the respiratory chain of Escherichia coli: an electrochemical and scanning electrochemical microscopy study of the antimicrobial mechanism of micromolar Ag+. , 2005, Biochemistry.

[8]  F. Pilati,et al.  Antibacterial activity of plastics coated with silver-doped organic-inorganic hybrid coatings prepared by sol-gel processes. , 2007, Biomacromolecules.

[9]  M. Rubner,et al.  Antibacterial properties of Ag nanoparticle loaded multilayers and formation of magnetically directed antibacterial microparticles. , 2005, Langmuir : the ACS journal of surfaces and colloids.

[10]  Chi-Ming Che,et al.  Proteomic analysis of the mode of antibacterial action of silver nanoparticles. , 2006, Journal of proteome research.

[11]  M. Yacamán,et al.  The bactericidal effect of silver nanoparticles , 2005, Nanotechnology.

[12]  T. Yogo,et al.  Synthesis of Dendrimer-Passivated Noble Metal Nanoparticles in a Polar Medium: Comparison of Size between Silver and Gold Particles , 2001 .

[13]  Xingmin Liu,et al.  One-step synthesis of biocompatible gold nanoparticles using gallic acid in the presence of poly-(N-vinyl-2-pyrrolidone) , 2007 .

[14]  S. Jeong,et al.  Antibacterial properties of padded PP/PE nonwovens incorporating nano-sized silver colloids , 2005 .

[15]  J. Song,et al.  Does the Antibacterial Activity of Silver Nanoparticles Depend on the Shape of the Nanoparticle? A Study of the Gram-Negative Bacterium Escherichia coli , 2007, Applied and Environmental Microbiology.

[16]  Carsten Sönnichsen,et al.  Plasmon resonances in large noble-metal clusters , 2002 .

[17]  S Person,et al.  Silver-coated nylon fiber as an antibacterial agent , 1987, Antimicrobial Agents and Chemotherapy.

[18]  G. Martínez-Castañón,et al.  Optical Absorption of Ag Particles Dispersed in a SiO2 Amorphous Matrix , 2005 .

[19]  Qingsheng Wu,et al.  Synergistic antibacterial effects of β-lactam antibiotic combined with silver nanoparticles , 2005 .

[20]  Arnab Roy,et al.  Characterization of enhanced antibacterial effects of novel silver nanoparticles , 2007, Nanotechnology.

[21]  M. Rubner,et al.  Two-level antibacterial coating with both release-killing and contact-killing capabilities. , 2006, Langmuir : the ACS journal of surfaces and colloids.