A fermentation process for the production of P50 from Serratia marcescens

Serratia marcescens es una enterobacteria gram-negativa oportunista aislada de las vias respiratorias y el tracto urinario en humanos. Entre las proteinas extracelulares segregadas de S. marcescens, esta la proteina P50 la que se produce en mayores cantidades y desempena un papel importante en la patogenesis de este microorganismo. Para producir esta proteina se ha estudiado un proceso de fermentacion. En primer lugar, en un diseno experimental, factorial de 23, se estudiaron cultivos suplementados con triptona, extracto de levadura y peptona. En bioreactores de 4L se estudio la influencia de la aireacion y la velocidad de agitacion sobre la produccion de la P50 en un diseno experimental 32. Por ultimo, se establecieron las condiciones optimas de crecimiento (28 °C, 400 rpm y 0.5 vvm), a esta escala, se obtuvo el 9,0 ODu/mL, 62% de nivel de expresion de la proteina P50 y 220 mg/L como la mayor produccion volumetrica de P50

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