Regeneration of whole meniscus using meniscal cells and polymer scaffolds in a rabbit total meniscectomy model.

The current treatments of meniscal lesion in knee joint are not perfect to prevent adverse effects of meniscus injury. Tissue engineering of meniscus using meniscal cells and polymer scaffolds could be an alternative option to treat meniscus injury. This study reports on the regeneration of whole medial meniscus in a rabbit total meniscectomy model using the tissue engineering technique. Biodegradable scaffolds in a meniscal shape were fabricated from polyglycolic acid (PGA) fiber meshes that were mechanically reinforced by bonding PGA fibers at cross points with 75:25 poly(lactic-co-glycolic acid). The compressive modulus of the bonded PGA scaffold was 28-fold higher than that of nonbonded scaffold. Allogeneic meniscal cells were isolated from rabbit meniscus biopsy and cultured in vitro. The expanded meniscal cells were seeded onto the polymer scaffolds, cultured in vitro for 1 week, and transplanted to rabbit knee joints from which medial menisci were removed. Ten or 36 weeks after transplantation, the implants formed neomenisci with the original scaffold shape maintained approximately. Hematoxylin and eosin staining of the sections of the neomenisci at 6 and 10 weeks revealed the regeneration of fibrocartilage. Safranin-O staining showed that abundant proteoglycan was present in the neomenisci at 10 weeks. Masson's trichrome staining indicated the presence of collagen. Immunohistochemical analysis showed that the presence of type I and II collagen in neomenisci at 10 weeks was similar to that of normal meniscal tissue. Biochemical and biomechanical analyses of the tissue-engineered menisci at 36 weeks were performed to determine the quality of the tissue-engineered menisci. Tissue-engineered meniscus showed differences in collagen content and aggregate modulus in comparison with native meniscus. This study demonstrates, for the first time, the feasibility of regenerating whole meniscal cartilage in a rabbit total meniscectomy model using the tissue engineering method.

[1]  W. W. Schaefer,et al.  The degenerative effects of partial and total resection of the medial meniscus in dogs' knees. , 1975, Clinical orthopaedics and related research.

[2]  D G Wilder,et al.  Mechanical changes in the knee after meniscectomy. , 1976, The Journal of bone and joint surgery. American volume.

[3]  A. Poole,et al.  Presence and mechanism of knee articular cartilage degeneration after meniscal reconstruction in dogs. , 2003, Osteoarthritis and cartilage.

[4]  R. Warren,et al.  Cellular repopulation of deep-frozen meniscal autografts: an experimental study in the dog. , 1992, Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association.

[5]  F. Guilak,et al.  Biomechanical factors in tissue engineered meniscal repair. , 1999, Clinical orthopaedics and related research.

[6]  S Tamai,et al.  Arthroscopic meniscal repair using fibrin glue. Part I: Experimental study. , 1997, Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association.

[7]  Rupal V Patel,et al.  Regional structural and viscoelastic properties of fibrocartilage upon dynamic nanoindentation of the articular condyle. , 2001, Journal of structural biology.

[8]  R Langer,et al.  Joint resurfacing using allograft chondrocytes and synthetic biodegradable polymer scaffolds. , 1994, Journal of biomedical materials research.

[9]  Pieter Buma,et al.  Tissue Engineering of the Meniscus , 2004 .

[10]  J. Steadman,et al.  Meniscal regeneration with copolymeric collagen scaffolds , 1992, The American journal of sports medicine.

[11]  J. Steadman,et al.  A clinical study of collagen meniscus implants to restore the injured meniscus. , 1999, Clinical orthopaedics and related research.

[12]  J. Vacanti,et al.  Tissue engineered meniscus: a potential new alternative to allogeneic meniscus transplantation. , 1997, Transplantation proceedings.

[13]  S. Arnoczky,et al.  Meniscal repair enhancement techniques. , 1996, Clinics in sports medicine.

[14]  K. Athanasiou,et al.  Toward tissue engineering of the knee meniscus. , 2001, Tissue engineering.

[15]  D. W. Jackson,et al.  Meniscal Repair Supplemented With Exogenous Fibrin Clot and Autogenous Cultured Marrow Cells in the Goat Model , 1996, The American journal of sports medicine.

[16]  W. Hayes,et al.  A mathematical analysis for indentation tests of articular cartilage. , 1972, Journal of biomechanics.

[17]  M. Spector,et al.  alpha-smooth muscle actin and contractile behavior of bovine meniscus cells seeded in type I and type II collagen-GAG matrices. , 1999, Journal of biomedical materials research.

[18]  C Perka,et al.  Tissue Engineered Cartilage Repair Using Cryopreserved and Noncryopreserved Chondrocytes , 2000, Clinical orthopaedics and related research.

[19]  Zhongnan Zhang,et al.  Repairs by Trephination and Suturing of Longitudinal Injuries in the Avascular Area of the Meniscus in Goats , 1995, The American journal of sports medicine.

[20]  A. Pennings,et al.  Meniscal tissue regeneration in porous 50/50 copoly(L-lactide/e-caprolactone) implants , 1997 .

[21]  Seedhom Bb.  Loadbearing function of the menisci. , 1976 .

[22]  J. Mansour,et al.  Immunochemical and Mechanical Characterization of Cartilage Subtypes in Rabbit , 2002, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[23]  M. Spector,et al.  Meniscus cells seeded in type I and type II collagen-GAG matrices in vitro. , 1999, Biomaterials.

[24]  R. Warren,et al.  Meniscal replacement using a cryopreserved allograft. An experimental study in the dog. , 1990, Clinical orthopaedics and related research.

[25]  K Athanasiou,et al.  Cytoindentation for obtaining cell biomechanical properties , 1999, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[26]  R. Warren,et al.  Meniscal injury and repair: clinical status. , 2000, The Orthopedic clinics of North America.

[27]  R Langer,et al.  Stabilized polyglycolic acid fibre-based tubes for tissue engineering. , 1996, Biomaterials.

[28]  A. Pennings,et al.  Porous implants for knee joint meniscus reconstruction: a preliminary study on the role of pore sizes in ingrowth and differentiation of fibrocartilage. , 1993, Clinical materials.

[29]  J. Steadman,et al.  Regeneration of Meniscal Cartilage with Use of a Collagen Scaffold. Analysis of Preliminary Data* , 1997, The Journal of bone and joint surgery. American volume.

[30]  R. Appleyard,et al.  Topographical analysis of the structural, biochemical and dynamic biomechanical properties of cartilage in an ovine model of osteoarthritis. , 2003, Osteoarthritis and cartilage.

[31]  A. M. Ahmed,et al.  In-vitro measurement of static pressure distribution in synovial joints--Part I: Tibial surface of the knee. , 1983, Journal of biomechanical engineering.

[32]  R. Warren,et al.  Tissue engineering meniscus: cells and matrix. , 2000, The Orthopedic clinics of North America.

[33]  D. Mooney,et al.  Engineered smooth muscle tissues: regulating cell phenotype with the scaffold. , 1999, Experimental cell research.

[34]  R. Warren,et al.  The effect of medial meniscectomy on anterior-posterior motion of the knee. , 1982, The Journal of bone and joint surgery. American volume.

[35]  M. Ochi,et al.  Immune response to fresh meniscal allografts in mice. , 1995, Journal of Surgical Research.

[36]  Seung‐Woo Cho,et al.  Engineering of volume-stable adipose tissues. , 2005, Biomaterials.

[37]  D J Mooney,et al.  Engineering smooth muscle tissue with a predefined structure. , 1998, Journal of biomedical materials research.