Cryptococcus cyanovorans sp. nov., a basidiomycetous yeast isolated from cyanide-contaminated soil.

Eighteen yeast strains were isolated and identified from cyanide-contaminated soil in South Africa. According to sequence-based analyses using the D1/D2 region of the large ribosomal subunit and ITS region, three of these strains were found to be identical and represent a novel species. Phylogenetic analysis based on the combined dataset of the D1/D2 and ITS regions revealed a grouping with Cryptococcus curvatus, representing a defined clade (Curvatus) in the order Trichosporonales. The three strains were demarcated from Cryptococcus curvatus by standard physiological tests such as assimilation of lactose, xylitol, 5-keto-D-gluconate, succinate and citrate as well as growth on media containing 10 % (w/v) NaCl and 5 % (w/v) glucose. In addition, it was established that these strains could utilize up to 10 mM NaCN as sole carbon source on solid media and as sole nitrogen source in liquid media. On the basis of these findings, it is suggested that the three strains represent a novel species for which the name Cryptococcus cyanovorans sp. nov. is given (type strain CBS 11948(T) = NRRL Y-48730(T)).

[1]  T. Boekhout,et al.  The Yeasts, a Taxonomic Study, 5th ed , 2011 .

[2]  Teun Boekhout,et al.  Cryptococcus Vuillemin (1901) , 2011 .

[3]  R. Bandoni,et al.  Discussion of Teleomorphic and Anamorphic Basidiomycetous Yeasts , 2011 .

[4]  L. C. Forti,et al.  Yeasts isolated from a fungus-growing ant nest, including the description of Trichosporon chiarellii sp. nov., an anamorphic basidiomycetous yeast. , 2010, International journal of systematic and evolutionary microbiology.

[5]  Ata Akcil,et al.  Biodegradation of cyanide containing effluents by Scenedesmus obliquus. , 2009, Journal of hazardous materials.

[6]  M. Takashima,et al.  Cryptococcus tepidarius sp. nov., a thermotolerant yeast species isolated from a stream from a hot-spring area in Japan. , 2009, International journal of systematic and evolutionary microbiology.

[7]  Mónica S. F. Santos,et al.  Removal of heavy metals using a brewer's yeast strain of Saccharomyces cerevisiae: the flocculation as a separation process. , 2008, Bioresource technology.

[8]  Rodrigo Lopez,et al.  Clustal W and Clustal X version 2.0 , 2007, Bioinform..

[9]  M. Nei,et al.  MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. , 2007, Molecular biology and evolution.

[10]  J. Albertyn,et al.  Cycloheximide resistance in the Lipomycetaceae revisited. , 2007, Canadian journal of microbiology.

[11]  A. Stolz,et al.  Isolation and characterization of a nitrile hydrolysing acidotolerant black yeast—Exophiala oligosperma R1 , 2007, Applied Microbiology and Biotechnology.

[12]  David T. Huang,et al.  Clarification of cyanide’s effect on oxygen transport characteristics in a canine model , 2007, Emergency Medicine Journal.

[13]  A. Casadevall,et al.  Cryptotrichosporon anacardii gen. nov., sp. nov., a new trichosporonoid capsulate basidiomycetous yeast from Nigeria that is able to form melanin on niger seed agar. , 2007, FEMS yeast research.

[14]  J. Albertyn,et al.  Cloning of an epoxide hydrolase‐encoding gene from Rhodotorula mucilaginosa and functional expression in Yarrowia lipolytica , 2007, Yeast.

[15]  J. Sampaio,et al.  Cryptococcus stepposus, a new filobasidiaceous yeast species found in the Prioksko-terrasny biosphere reserve in Russia. , 2006, Mycological research.

[16]  J. Lynch,et al.  Biodegradation of cyanide by Trichoderma spp. and Fusarium spp. , 2005 .

[17]  G. Scorzetti,et al.  Systematics of the anamorphic basidiomycetous yeast genus Trichosporon Behrend with the description of five novel species: Trichosporon vadense, T. smithiae, T. dehoogii, T. scarabaeorum and T. gamsii. , 2004, International journal of systematic and evolutionary microbiology.

[18]  C. Kurtzman,et al.  Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences , 1998, Antonie van Leeuwenhoek.

[19]  Ata Akcil,et al.  Destruction of cyanide in gold mill effluents: biological versus chemical treatments. , 2003, Biotechnology advances.

[20]  G. Scorzetti,et al.  Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions. , 2002, FEMS yeast research.

[21]  J. M. Park,et al.  Degradation of tetracyanonickelate (II) by Cryptococcus humicolus MCN2. , 2002, FEMS microbiology letters.

[22]  J. Trenkler,et al.  MR changes after acute cyanide intoxication. , 2002, AJNR. American journal of neuroradiology.

[23]  M. Takashima,et al.  Reclassification of the Cryptococcus humicola complex. , 2001, International journal of systematic and evolutionary microbiology.

[24]  V. Ferraz,et al.  Metabolism of benzonitrile by Cryptococcus sp. UFMG‐Y28 , 2000, Journal of basic microbiology.

[25]  Ramaraj Boopathy,et al.  Factors limiting bioremediation technologies , 2000, Bioresource Technology.

[26]  T. Boekhout,et al.  Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. , 2000, International journal of systematic and evolutionary microbiology.

[27]  Peter Kjeldsen,et al.  Behaviour of Cyanides in Soil and Groundwater: A Review , 1999 .

[28]  D. Boening,et al.  A critical review: general toxicity and environmental fate of three aqueous cyanide ions and associated ligands , 1999 .

[29]  C. Knowles,et al.  Metabolism and enzymology of cyanide/metallocyanide biodegradation by Fusarium solani under neutral and acidic conditions , 1998 .

[30]  Faizal Bux,et al.  Considerations for application of biosorption technology to remediate metal-contaminated industrial effluents , 1998 .

[31]  D. Yarrow.  Methods for the isolation, maintenance and identification of yeasts , 1998 .

[32]  T. Choné,et al.  Cyanide Degradation under Alkaline Conditions by a Strain of Fusarium solani Isolated from Contaminated Soils , 1997, Applied and environmental microbiology.

[33]  M. Wingfield,et al.  Ultrastructure of ascus arrangement and ascospore development in Ophiostoma seticolle , 1994 .

[34]  Yuzo Yamada,et al.  Molecular approaches to the taxonomy of ballistosporous yeasts based on the analysis of the partial nucleotide sequences of 18S ribosomal ribonucleic acids. , 1993 .

[35]  M. Adams.  The removal of cyanide from aqueous solution by the use of ferrous sulphate , 1992 .

[36]  T. White.  Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics , 1990 .

[37]  J. P. Walt,et al.  Chapter II – Methods for the isolation, maintenance, classification and identification of yeasts , 1984 .