Odor response properties of rat olfactory receptor neurons.

Molecular biology studies of olfaction have identified a multigene family of molecular receptors that are likely to be involved in odor transduction mechanisms. However, because previous functional data on peripheral coding were mainly collected from inferior vertebrates, it has been difficult to document the degree of specificity of odor interaction mechanisms. As a matter of fact, studies of the functional expression of olfactory receptors have not demonstrated the low or high specificity of olfactory receptors. In this study, the selectivity of olfactory receptor neurons was investigated in the rat at the cellular level under physiological conditions by unitary extracellular recordings. Individual olfactory receptor neurons were broadly responsive to qualitatively distinct odor compounds. We conclude that peripheral coding is based on activated arrays of olfactory receptor cells with overlapping tuning profiles.

[1]  A. Duchamp,et al.  ODOR DISCRIMINATION BY FROG OLFACTORY RECEPTORS , 1974 .

[2]  Charles D. Sigwart,et al.  Olfactory receptor units—a mammalian preparation , 1977, Brain Research.

[3]  R. Gesteland,et al.  Development of olfactory receptor neuron selectivity in the rat fetus , 1982, Neuroscience.

[4]  Michel Vigouroux,et al.  A wide concentration range olfactometer for delivery of short reproducible odor pulses , 1988, Journal of Neuroscience Methods.

[5]  P Duchamp-Viret,et al.  Amplifying role of convergence in olfactory system a comparative study of receptor cell and second-order neuron sensitivities. , 1989, Journal of neurophysiology.

[6]  T. A. Harrison,et al.  Discrimination among odorants by single neurons of the rat olfactory bulb. , 1989, Journal of neurophysiology.

[7]  G. Sicard,et al.  Olfactory discrimination over a wide concentration range. Comparison of receptor cell and bulb neuron abilities , 1990, Brain Research.

[8]  R. Axel,et al.  A novel multigene family may encode odorant receptors: A molecular basis for odor recognition , 1991, Cell.

[9]  H. Breer,et al.  Expression of odorant receptors in spatially restricted subsets of chemosensory neurones. , 1992, Neuroreport.

[10]  S. Heinemann,et al.  Spatial pattern of receptor expression in the olfactory epithelium. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[11]  H. Breer,et al.  Cloning and expression of odorant receptors , 1993, Nature.

[12]  Linda B. Buck,et al.  A zonal organization of odorant receptor gene expression in the olfactory epithelium , 1993, Cell.

[13]  L. Buck.  Identification and analysis of a multigene family encoding odorant receptors: implications for mechanisms underlying olfactory information processing , 1993 .

[14]  Richard Axel,et al.  Topographic organization of sensory projections to the olfactory bulb , 1994, Cell.

[15]  M. Tonoike,et al.  Tuning specificities to aliphatic odorants in mouse olfactory receptor neurons and their local distribution. , 1994, Journal of neurophysiology.

[16]  P. Emson,et al.  Cellular localization of putative odorant receptor mRNAs in olfactory and chemosensory neurons: a non radioactive in situ hybridization study. , 1994, Brain research. Molecular brain research.

[17]  Yoshihiro Yoshihara,et al.  Molecular recognition and olfactory processing in the mammalian olfactory system , 1995, Progress in Neurobiology.

[18]  Patricia Duchamp-Viret,et al.  Odor processing in the frog olfactory system , 1997, Progress in Neurobiology.

[19]  J. Kauer,et al.  Odorant Response Properties of Convergent Olfactory Receptor Neurons , 1998, The Journal of Neuroscience.

[20]  M. Chalansonnet,et al.  Olfactory bulb output cell temporal response patterns to increasing odor concentrations in freely breathing rats. , 1998, Chemical senses.

[21]  K. Mikoshiba,et al.  Functional expression of a mammalian odorant receptor. , 1998, Science.

[22]  R. Axel,et al.  Expression of candidate pheromone receptor genes in vomeronasal neurons. , 1998, Chemical senses.

[23]  L. Buck,et al.  Combinatorial Receptor Codes for Odors , 1999, Cell.