The gene expression level of p53 and p21 in mouse brain exposed to radiofrequency field

Background : Widespread and growing sources of electromagnec radiaon raised concerns aributed to the potenal adverse h ealth risk of radiofrequency fields. Given the funconal importance of the hippocampus, this study aimed to invesgate the effects of electromagnec waves radiated by mobile jammer on hippocampal expression of p21 and p53 genes as regulators of cellular apoptosis. Materials and Methods : Forty-eight male BALB/c mice were randomly divided into six groups (n=8 each). Animals in the experimental groups were radiated at the frequencies of 900 and 1800 megahertz for a period of 30 consecuve days, while the control group remains constant during the experiment. The hippocampal expression of p21and p53 mRNAs were evaluated using Real-Time PCR. Results: There were not differences between the mean expression level of p53 and p21 genes of the exposure groups compared to those of the control group (P>0.05). The rao expression of p53 and p21 genes was increased to greater than one (p53/p21>1) in almost all experimental groups compared to controls. However, there was not significant differences between the expression level of p53 and p21 genes among the experimental groups using paired t test (p>0.05). Conclusion: Taken together, our findings demonstrate changes in hippocampal expression level of p53 and p21 a;er mo bile jammer radiaon. However, cell condion expected to remain relavely stable over the exposure period due to parallel changes of both pro- and an�- apoptoc genes at the sameme.