Effect of acupuncture on behavioral hyperactivity and dopamine release in the nucleus accumbens in rats sensitized to morphine

Acupuncture as a therapeutic intervention has been used for the treatment of many functional disorders including substance abuse. However, there are still many unanswered question about the basic mechanism underlying acupuncture's effectiveness in the treatment of drug addiction. Repeated injection of psycostimulants or morphine can produce behavioral and neurochemical sensitization and have been used as a model for studying drug addiction. The present study was designed to investigate the effect of acupuncture on repeated morphine-induced changes in extracellular dopamine levels using in vivo microdialysis and repeated morphine-induced behavioral changes. Male Sprague-Dawley rats were treated with saline or increasing doses of morphine (10, 20 and 40 mg/kg, s.c., twice daily for 3 days). Following 15 days of withdrawal, acupuncture was applied at bilateral Shenmen (HT7) points for 1 min after the systemic challenge with morphine HCl (5 mg/kg, s.c.). Results showed that acupuncture at the specific acupoint HT7, but not at control points (TE8 and tail) significantly decreased both dopamine release in the nucleus accumbens and behavioral hyperactivity induced by a systemic morphine challenge. These results suggest that the therapeutic effect of acupuncture on morphine addiction occurs through inhibition of neurochemical and behavioral sensitization to morphine.

[1]  K. Berridge,et al.  Incentive-sensitization and addiction. , 2001, Addiction.

[2]  G. Paxinos,et al.  The Rat Brain in Stereotaxic Coordinates , 1983 .

[3]  G. Kaplan,et al.  GABAB Receptor Activation in the Ventral Tegmental Area Inhibits the Acquisition and Expression of Opiate-Induced Motor Sensitization , 2004, Journal of Pharmacology and Experimental Therapeutics.

[4]  C. Yang,et al.  Acupuncture attenuates repeated nicotine-induced behavioral sensitization and c-Fos expression in the nucleus accumbens and striatum of the rat , 2004, Neuroscience Letters.

[5]  G. Chiara,et al.  Reciprocal changes in dopamine responsiveness in the nucleus accumbens shell and core and in the dorsal caudate–putamen in rats sensitized to morphine , 1999, Neuroscience.

[6]  T. Kosten,et al.  Acupuncture for the treatment of cocaine addiction. Investigation of a needle puncture control. , 1995, Journal of substance abuse treatment.

[7]  F. Luo,et al.  Peripheral electric stimulation inhibits morphine‐induced place preference in rats , 2000, Neuroreport.

[8]  A. M. Schoen.  Veterinary Acupuncture: Ancient Art to Modern Medicine , 2001 .

[9]  G. Di Chiara,et al.  Drugs abused by humans preferentially increase synaptic dopamine concentrations in the mesolimbic system of freely moving rats. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[10]  C. Hodge,et al.  Microdialysis in the mouse nucleus accumbens: a method for detection of monoamine and amino acid neurotransmitters with simultaneous assessment of locomotor activity. , 2000, Brain research. Brain research protocols.

[11]  L. Vanderschuren,et al.  A single exposure to morphine induces long‐lasting behavioural and neurochemical sensitization in rats , 2001, The European journal of neuroscience.

[12]  H. Lee,et al.  New findings of the correlation between acupoints and corresponding brain cortices using functional MRI. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[13]  G. Koob.  Drugs of abuse: anatomy, pharmacology and function of reward pathways. , 1992, Trends in pharmacological sciences.

[14]  Electroacupuncture stimulation suppresses the increase in alcohol-drinking behavior in restricted rats. , 2001 .

[15]  Hi-Joon Park,et al.  Acupuncture stimulation of the vision-related acupoint (Bl-67) increases c-Fos expression in the visual cortex of binocularly deprived rat pups. , 2002, The American journal of Chinese medicine.

[16]  Dr. med. Gabriel Stux,et al.  ACUPUNCTURE , 1974, Springer Berlin Heidelberg.

[17]  K. A. Trujillo,et al.  Continuous administration of opioids produces locomotor sensitization , 2004, Pharmacology Biochemistry and Behavior.

[18]  P. Kalivas,et al.  Alterations in dopaminergic and glutamatergic transmission in the induction and expression of behavioral sensitization: a critical review of preclinical studies , 2000, Psychopharmacology.

[19]  Jisheng Han,et al.  Suppression of morphine withdrawal by electroacupuncture in rats: dynorphin and κ-opioid receptor implicated , 1999, Brain Research.

[20]  M. Cousins,et al.  GABA(B) receptor agonists for the treatment of drug addiction: a review of recent findings. , 2002, Drug and alcohol dependence.

[21]  Yinli Lin.  Acupuncture treatment for insomnia and acupuncture analgesia , 1995, Psychiatry and clinical neurosciences.

[22]  C. Yang,et al.  Acupuncture-mediated inhibition of ethanol-induced dopamine release in the rat nucleus accumbens through the GABAB receptor , 2004, Neuroscience Letters.

[23]  R. E. Sherman,et al.  A large randomized placebo controlled study of auricular acupuncture for alcohol dependence. , 2002, Journal of substance abuse treatment.

[24]  J. Konefal,et al.  Acupuncture for the treatment of cocaine addiction: a randomized controlled trial. , 2002, JAMA.

[25]  G. Chiara.  The role of dopamine in drug abuse viewed from the perspective of its role in motivation , 1995 .

[26]  Hi-Joon Park,et al.  Acupuncture increases neuropeptide Y expression in hippocampus of maternally-separated rats , 2003, Neuroscience Letters.