Efficient resolution of prostereogenic arylaliphatic ketones using a recombinant alcohol dehydrogenase from Pseudomonas fluorescens

Abstract A broad range of arylaliphatic ketones is efficiently reduced to the corresponding optically active ( R )-alcohols by a recombinant alcohol dehydrogenase from Pseudomonas fluorescens (PFADH) produced by overexpression in Escherichia coli. PFADH shows high activity and stereoselectivity in the reduction of acetophenone and various derivatives (45–99% e.e.), as well as in the reduction of 3-oxobutyric acid methyl ester (>99% e.e.). The highest activity was observed between 10 and 20°C. The cofactor NADH can be efficiently recycled by the addition of 10–20% (v/v) of iso -propanol.

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