A COMPARISON OF HEAT-INDUCED CHANGES OF INTRAMUSCULAR CONNECTIVE TISSUE AND COLLAGEN OF BEEF SEMITENDINOSUS MUSCLE DURING WATER BATH AND MICROWAVE HEATING

The main objective of this study was to characterize the thermal properties of intramuscular connective tissue (IMCT) and collagen in beef semitendinosus (ST) muscle from Chinese yellow bulls during water bath and microwave heating. Beef ST muscle was heated to internal endpoint temperature of 40, 50, 60, 70, 80 and 90C by water bath and microwave oven, respectively. The results indicated that insoluble collagen contents of microwave-heated meat were higher than water bath heated except for 90C, and showed significant differences at 80 and 90C (P < 0.05). Mechanical strength of IMCT showed significant difference (P < 0.05) at 60C between water bath- and microwave-heated meat. Perimysial portion content of microwave-heated meat was significantly higher than water bath heated at 60, 70, 80 (P < 0.05) and 90°C (P < 0.01), and endomysial portion content showed a gradual decrease during heating. A heating temperature of 60C was critical, and affects the maximum shrinkage temperatures (Tmax) of IMCT for both heated meat. With the increase in heating temperature, granulation changes of IMCT and disorderly changes of collagenous fibers arrangement have resulted. PRACTICAL APPLICATIONS It is well known that the characteristics of intramuscular connective tissue (IMCT) and collagen are related to meat quality, especially for the meat tenderness. Besides, heating is an important step for the conversion of inedible (raw) meat to edible meat, and can make remarkable effects on the eating quality of meat. Based on those viewpoints, we reported the comparative research of water bath and microwave heating on the effects of meat quality and thermal characteristics of IMCT and collagen. Results reported in this article would be useful either for the science community or for the food industry of beef production. Moreover, our study can provide the reference for the systematic researches for Chinese yellow bulls in the future.

[1]  E. Dransfield,et al.  Perimysial collagen crosslinking and meat tenderness in Belgian Blue double-muscled cattle. , 2002, Meat science.

[2]  M. Kaya,et al.  Influence of weak organic acids and salts on the denaturation characteristics of intramuscular connective tissue. A differential scanning calorimetry study. , 2001, Meat science.

[3]  S. Tabata,et al.  Relationship among collagen amount, distribution and architecture in the M. longissimus thoracis and M. pectoralis profundus from pigs. , 2003, Meat science.

[4]  J. Savell,et al.  Perimysium thickness as an indicator of beef tenderness. , 2004, Meat science.

[5]  P. Purslow Intramuscular connective tissue and its role in meat quality. , 2005, Meat science.

[6]  C. Vasanthi,et al.  Effect of cooking temperature and time on the physico-chemical, histological and sensory properties of female carabeef (buffalo) meat. , 2007, Meat science.

[7]  Allen J. Bailey,et al.  Connective Tissue in Meat and Meat Products , 1989 .

[8]  X. Xu,et al.  Comparisons of meat quality characteristics and intramuscular connective tissue between beef longissimus dorsi and semitendinosus muscles from chinese yellow bulls , 2007 .

[9]  T. Nishimura,et al.  Structural changes in intramuscular connective tissue during the fattening of Japanese black cattle: effect of marbling on beef tenderization. , 1999, Journal of animal science.

[10]  G. Höhne,et al.  Differential Scanning Calorimetry , 2007 .

[11]  S. Velleman The role of the extracellular matrix in skeletal muscle development. , 1999, Poultry science.

[12]  N. Light,et al.  Characterization of muscle epimysium, perimysium and endomysium collagens. , 1984, The Biochemical journal.

[13]  M. Sacks,et al.  Contribution of Intramuscular Connective Tissue to the Viscoelastic Properties of Post‐Rigor Bovine Muscle , 1988 .

[14]  J. Kijowski,et al.  Thermal Properties of Proteins in Chicken Broiler Tissues , 1988 .

[15]  B. Giménez,et al.  Shear values of raw samples of 14 bovine muscles and their relation to muscle collagen characteristics. , 2003, Meat science.

[16]  J. Hill The Solubility of Intramuscular Collagen in Meat Animals of Various Ages , 1966 .

[17]  J. Crouse,et al.  The relationship of muscle fibre size to tenderness of beef. , 1991, Meat science.

[18]  P. V. Harris,et al.  Effect of Cooking Temperature and Time on the Shear Properties of Meat , 1981 .

[19]  M. Ruusunen,et al.  Comparison of the thermal characteristics of connective tissue in loose structured and normal structured porcine M. semimembranosus. , 2008, Meat science.

[20]  S. Tabata,et al.  Developmental states of the collagen content, distribution and architecture in the pectoralis, iliotibialis lateralis and puboischiofemoralis muscles of male Red Cornish × New Hampshire and normal broilers , 2004, British poultry science.

[21]  D. E. Goll,et al.  Age-associated changes in muscle composition. The isolation and properties of a collagenous residue from bovine muscle. , 1963 .

[22]  P. B. Lynch,et al.  Effects of PSE on the quality of cooked hams. , 2003, Meat science.

[23]  A. Bailey,et al.  The rôle of epimysial, perimysial and endomysial collagen in determining texture in six bovine muscles. , 1985, Meat science.

[24]  D. Ledward,et al.  Effect of heat treatment on changes in texture, structure and properties of Thai indigenous chicken muscle , 2005 .

[25]  E. Dransfield Intramuscular composition and texture of beef muscles , 1977 .

[26]  R. E. Neuman,et al.  The determination of hydroxyproline. , 1950, The Journal of biological chemistry.

[27]  P. Purslow,et al.  The effect of cooking temperature on mechanical properties of whole meat, single muscle fibres and perimysial connective tissue. , 2000, Meat science.

[28]  K. Palka Changes in intramuscular connective tissue and collagen solubility of bovine m.semitendinosus during retorting. , 1999, Meat science.

[29]  X. Xu,et al.  Dynamical Changes of Beef Intramuscular Connective Tissue and Muscle Fiber during Heating and their Effects on Beef Shear Force , 2010 .

[30]  I. Oshima,et al.  Comparative study of the histochemical properties, collagen content and architecture of the skeletal muscles of wild boar crossbred pigs and commercial hybrid pigs. , 2009, Meat science.

[31]  T. Nishimura,et al.  Relationship between development of intramuscular connective tissue and toughness of pork during growth of pigs. , 1999, Journal of animal science.

[32]  M. Dikeman,et al.  Tenderness and collagen composition of beef semitendinosus roasts cooked by conventional convective cooking and modeled, multi-stage, convective cooking. , 2000, Meat science.

[33]  T K Borg,et al.  Morphology of connective tissue in skeletal muscle. , 1980, Tissue & cell.

[34]  K. Palka The influence of post-mortem ageing and roasting on the microstructure, texture and collagen solubility of bovine semitendinosus muscle. , 2003, Meat science.

[35]  I. Bergman,et al.  Two Improved and Simplified Methods for the Spectrophotometric Determination of Hydroxyproline. , 1963 .