Thermally sensitive dual fluorescent polymeric micelles for probing cell properties

Dual fluorescent micelles with a hydrophobic probe (HMA) embedded in the micelle core and a hydrophilic probe (TRITC) attached on the micelle corona were prepared. These micelles can act as nanometre-sized thermal sensors. Within a short temperature range, the fluorescent emission of the micelles changes strongly and reversibly as a function of temperature. These micelles can be easily taken up by human HeLa cells and can potentially be used as intracellular thermometers.

[1]  M. C. Stuart,et al.  Mobility of fluorescently labeled polymer micelles in living cells , 2011 .

[2]  Chao-Tsen Chen,et al.  A PNIPAM-based fluorescent nanothermometer with ratiometric readout. , 2011, Chemical communications.

[3]  Jinming Hu,et al.  Responsive Polymers for Detection and Sensing Applications: Current Status and Future Developments , 2010 .

[4]  M. C. Stuart,et al.  Pluronic polymersomes stabilized by core cross-linked polymer micelles , 2009 .

[5]  Y. Harada,et al.  Hydrophilic fluorescent nanogel thermometer for intracellular thermometry. , 2009, Journal of the American Chemical Society.

[6]  Gaurav Sahay,et al.  Amphiphilic block copolymers enhance cellular uptake and nuclear entry of polyplex-delivered DNA. , 2008, Bioconjugate chemistry.

[7]  Dieter Braun,et al.  Why molecules move along a temperature gradient , 2006, Proceedings of the National Academy of Sciences.

[8]  A. Müller,et al.  Stabilization of polymeric micelles with a mixed poly(ethylene oxide)/poly(2-hydroxyethyl methacrylate) shell by formation of poly(pentaerythritol tetraacrylate) nanonetworks within the micelles , 2006 .

[9]  H. Hamann,et al.  Ultra-high-density phase-change storage and memory , 2006, Nature materials.

[10]  R. Fischer,et al.  One-step analysis of protein complexes in microliters of cell lysate , 2005, Nature Methods.

[11]  H. Dai,et al.  Carbon nanotubes as multifunctional biological transporters and near-infrared agents for selective cancer cell destruction. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[12]  P. Petrov,et al.  Innovative approach for stabilizing poly(ethylene oxide)-b-poly(propylene oxide)-b-poly(ethylene oxide) micelles by forming nano-sized networks in the micelle , 2005 .

[13]  M. W. George,et al.  Quantitative analysis of the formation and diffusion of A1-adenosine receptor-antagonist complexes in single living cells. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[14]  R. Stafford,et al.  Nanoshell-mediated near-infrared thermal therapy of tumors under magnetic resonance guidance , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[15]  F. Bates,et al.  Polymer vesicles in vivo: correlations with PEG molecular weight. , 2003, Journal of controlled release : official journal of the Controlled Release Society.

[16]  Donald W. Miller,et al.  Optimal Structure Requirements for Pluronic Block Copolymers in Modifying P-glycoprotein Drug Efflux Transporter Activity in Bovine Brain Microvessel Endothelial Cells , 2003, Journal of Pharmacology and Experimental Therapeutics.

[17]  Heather Knight,et al.  Temperature sensing by plants: the primary characteristics of signal perception and calcium response. , 1999, The Plant journal : for cell and molecular biology.

[18]  Kazuo Maruyama,et al.  Amphipathic polyethyleneglycols effectively prolong the circulation time of liposomes , 1990, FEBS letters.

[19]  M. C. Stuart,et al.  Stabilization of Polymersome Vesicles by an Interpenetrating Polymer Network , 2007 .

[20]  Yechezkel Barenholz,et al.  Pharmacokinetics of Pegylated Liposomal Doxorubicin , 2003, Clinical pharmacokinetics.