Cross-linked lysozyme crystal templated synthesis of Au nanoparticles as high-performance recyclable catalysts

Bio-nanomaterials fabricated using a bioinspired templating technique represent a novel class of composite materials with diverse applications in biomedical, electronic devices, drug delivery, and catalysis. In this study, Au nanoparticles (NPs) are synthesized within the solvent channels of cross-linked lysozyme crystals (CLLCs) in situ without the introduction of extra chemical reagents or physical treatments. The as-prepared AuNPs-in-protein crystal hybrid materials are characterized by light microscopy, transmission electron microscopy, x-ray diffraction, and Fourier-transform infrared spectroscopy analyses. Small AuNPs with narrow size distribution reveal the restriction effects of the porous structure in the lysozyme crystals. These composite materials are proven to be active heterogeneous catalysts for the reduction of 4-nitrophenol to 4-aminophenol. These catalysts can be easily recovered and reused at least 20 times because of the physical stability and macro-dimension of CLLCs. This work is the first to use CLLCs as a solid biotemplate for the preparation of recyclable high-performance catalysts.

[1]  O. Muskens,et al.  Plasmonic Response of Ag‐ and Au‐Infiltrated Cross‐Linked Lysozyme Crystals , 2013 .

[2]  Yaqi Cai,et al.  A novel Fe3O4–graphene–Au multifunctional nanocomposite: green synthesis and catalytic application , 2012 .

[3]  L. Liz‐Marzán,et al.  Catalysis by metallic nanoparticles in aqueous solution: model reactions. , 2012, Chemical Society reviews.

[4]  Xiao Yan,et al.  In situ auto-reduction of silver nanoparticles in mesoporous carbon with multifunctionalized surfaces , 2012 .

[5]  S. Kitagawa,et al.  Porous protein crystals as reaction vessels for controlling magnetic properties of nanoparticles. , 2012, Small.

[6]  C. Dickinson,et al.  Synthesis, characterization and catalytic activity of gold nanoparticles biosynthesized with Rhizopus oryzae protein extract , 2012 .

[7]  Chun-yan Liu,et al.  Au/graphene hydrogel: synthesis, characterization and its use for catalytic reduction of 4-nitrophenol , 2012 .

[8]  Yi Lu,et al.  Catalysis of gold nanoparticles within lysozyme single crystals. , 2012, Chemistry, an Asian journal.

[9]  Chengzhou Zhu,et al.  In situ loading of well-dispersed gold nanoparticles on two-dimensional graphene oxide/SiO2 composite nanosheets and their catalytic properties. , 2012, Nanoscale.

[10]  J. Luong,et al.  Catalysis using gold nanoparticles decorated on nanocrystalline cellulose. , 2012, Nanoscale.

[11]  Yi Lu,et al.  Catalysis of Gold Nanoparticles within Lysozyme Single Crystals , 2012 .

[12]  W. Qi,et al.  Shape evolution and thermal stability of lysozyme crystals: effect of pH and temperature , 2012, Bioprocess and Biosystems Engineering.

[13]  R. Arakawa,et al.  ph‐Dependent Synthesis of Pepsin‐Mediated Gold Nanoclusters with Blue Green and Red Fluorescent Emission , 2011 .

[14]  Yichun Liu,et al.  In situ assembly of well-dispersed Ag nanoparticles (AgNPs) on electrospun carbon nanofibers (CNFs) for catalytic reduction of 4-nitrophenol. , 2011, Nanoscale.

[15]  N. Ghosh,et al.  Synthesis of Ag nanoparticles within the pores of SBA-15: An efficient catalyst for reduction of 4-nitrophenol , 2011 .

[16]  B. Shi,et al.  Highly dispersed heterogeneous palladium catalysts by the introduction of plant tannin into porous Al2O3 supports , 2011 .

[17]  Marc Schneider,et al.  Formation of Fluorescent Metal (Au, Ag) Nanoclusters Capped in Bovine Serum Albumin Followed by Fluorescence and Spectroscopy , 2011 .

[18]  R. Doong,et al.  Bifunctional Au−Fe3O4 Heterostructures for Magnetically Recyclable Catalysis of Nitrophenol Reduction , 2011 .

[19]  Yichun Liu,et al.  In situ assembly of well-dispersed gold nanoparticles on electrospun silica nanotubes for catalytic reduction of 4-nitrophenol. , 2011, Chemical communications.

[20]  H. Wu,et al.  Polyphenol-grafted collagen fiber as reductant and stabilizer for one-step synthesis of size-controlled gold nanoparticles and their catalytic application to 4-nitrophenol reduction , 2011 .

[21]  Yi Lu,et al.  Time-dependent, protein-directed growth of gold nanoparticles within a single crystal of lysozyme. , 2011, Nature nanotechnology.

[22]  B. Shi,et al.  Synthesis of highly active and reusable supported gold nanoparticles and their catalytic applications to 4-nitrophenol reduction , 2011 .

[23]  J. Mccammon,et al.  Porous protein frameworks with unsaturated metal centers in sterically encumbered coordination sites. , 2011, Chemical communications.

[24]  Younan Xia,et al.  Aqueous‐Phase Synthesis of Pt/CeO2 Hybrid Nanostructures and Their Catalytic Properties , 2010, Advanced materials.

[25]  Yi Lu,et al.  Lysozyme-stabilized gold fluorescent cluster: Synthesis and application as Hg(2+) sensor. , 2010, The Analyst.

[26]  Hongyuan Wei,et al.  Crystallization control of thermal stability and morphology of hen egg white lysozyme crystals by ionic liquids. , 2010, Journal of agricultural and food chemistry.

[27]  Koichiro Tanaka,et al.  Modification of porous protein crystals in development of biohybrid materials. , 2010, Bioconjugate chemistry.

[28]  S. Mann,et al.  Template-directed synthesis of nanoplasmonic arrays by intracrystalline metalization of cross-linked lysozyme crystals. , 2010, Angewandte Chemie.

[29]  Jianping Xie,et al.  Protein-directed synthesis of highly fluorescent gold nanoclusters. , 2009, Journal of the American Chemical Society.

[30]  A. Matzger,et al.  Selection of Protein Crystal Forms Facilitated by Polymer-Induced Heteronucleation. , 2008, Crystal growth & design.

[31]  E. Vlieg,et al.  Growth Inhibition of Protein Crystals : A Study of Lysozyme Polymorphs , 2008 .

[32]  Jianping Xie,et al.  Silver nanoplates: from biological to biomimetic synthesis. , 2007, ACS nano.

[33]  Vincent Artero,et al.  Tricarbonylmanganese(I)-lysozyme complex: a structurally characterized organometallic protein. , 2007, Chemical communications.

[34]  E. Weckert,et al.  The structure of the hexagonal crystal form of hen egg-white lysozyme. , 2006, Acta crystallographica. Section D, Biological crystallography.

[35]  Qinghua Zhang,et al.  From CO oxidation to CO2 activation: an unexpected catalytic activity of polymer-supported nanogold. , 2005, Journal of the American Chemical Society.

[36]  A. Straathof,et al.  Adsorption of xanthene dyes by lysozyme crystals. , 2005, Langmuir : the ACS journal of surfaces and colloids.

[37]  A. Margolin,et al.  Cross-Linked Enzyme Crystals as Highly Active Catalysts in Organic Solvents , 1996 .

[38]  Hiroshi Sano,et al.  Novel Gold Catalysts for the Oxidation of Carbon Monoxide at a Temperature far Below 0 °C , 1987 .