Comparative proteomic profiling of human lung adenocarcinoma cells (CL 1–0) expressing miR‐372

Lung cancer is a common malignancy and has a poor overall prognosis. Widespread metastasis is a common phenomenon in non‐small cell lung cancer (NSCLC). It has been demonstrated that cancer relapse and survival can be predicted by the presence of a five‐microRNA (miRNA) signature independent of stage or histologic type in NSCLC patients. Among the five miRNAs in the signature, miR‐372 has been shown to play a significant role in metastasis and in the development of human testicular germ cell tumors. In addition, there is evidence that miR‐372 posttranscriptionally downregulates large tumor suppressor, homolog 2 (Lats2), resulting in tumorigenesis and proliferation. To further investigate the cellular mechanisms involved in miR‐372‐induced silencing, we conducted a comparative proteomic analysis of NSCLC CL 1–0 cells expressing miRNA‐372 and/or vector only by using two‐dimensional gel electrophoresis (2DE), two‐dimensional difference gel electrophoresis (2D‐DIGE), and LC/MS/MS. Proteins identified as being up‐ or downregulated were further classified according to their biological functions. Many of the proteins identified in our study may be potential diagnostic biomarkers of NSCLC, particularly phosphorylated eIF4A‐I.

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