A disposable amperometric ethanol biosensor based on screen-printed carbon electrodes mediated with ferricyanide-magnetic nanoparticle mixture

Abstract Magnetic Fe 3 O 4 nanoparticles were prepared by co-precipitation method and used to develop a reagentless disposable amperometric ethanol (EtOH) biosensor. The electrochemical characteristics of modified processes were analyzed by cyclic voltammetry (CV) and chronoamperometry (CA). Results showed that the presence of Fe 3 O 4 nanoparticles could enhance the peak currents of redox species. Moreover, the alcohol biosensor exhibited an excellent sensitivity and fast response time for EtOH with a wide linear response range from 1.0 to 9.0 mM.

[1]  P. Blanco,et al.  Amperometric detection of ethanol with poly-(o-phenylenediamine)-modified enzyme electrodes , 1997 .

[2]  Dong-Hwang Chen,et al.  Immobilization of yeast alcohol dehydrogenase on magnetic nanoparticles for improving its stability , 2001, Biotechnology Letters.

[3]  Dongfang Cao,et al.  Electrochemical biosensors utilising electron transfer in heme proteins immobilised on Fe3O4 nanoparticles. , 2003, The Analyst.

[4]  Itamar Willner,et al.  Electroanalytical and Bioelectroanalytical Systems Based on Metal and Semiconductor Nanoparticles , 2004 .

[5]  Zeev Rosenzweig,et al.  Glucose oxidase–magnetite nanoparticle bioconjugate for glucose sensing , 2004, Analytical and bioanalytical chemistry.

[6]  L. Gorton,et al.  Reagentless chemically modified carbon paste electrode based on a phenothiazine polymer derivative and yeast alcohol dehydrogenase for the analysis of ethanol , 1993 .

[7]  Mihaela Niculescu,et al.  Quinohemoprotein alcohol dehydrogenase-based reagentless amperometric biosensor for ethanol monitoring during wine fermentation , 2002 .

[8]  Lauro T. Kubota,et al.  Highly stable amperometric biosensor for ethanol based on Meldola's blue adsorbed on silica gel modified with niobium oxide , 2003 .

[9]  A. Reshetilov,et al.  Characteristics of Gluconobacter oxydans B-1280 and Pichia methanolica MN4 cell based biosensors for detection of ethanol , 2001 .

[10]  C. Cai,et al.  Amperometric biosensor for ethanol based on immobilization of alcohol dehydrogenase on a nickel hexacyanoferrate modified microband gold electrode. , 1997, Talanta.