Quantitative detection of Escherichia coli and measurement of urinary tract infection diagnosis possibility by use of a portable, handheld sensor

Electrochemical nitrite sensor was used to quantitatively detect the nitrite concentration in urine and by building the approximate relationship between the nitrite concentration and number of E.coli bacterias, electrochemical nitrite sensor can count the numbers of Escherichia coli and do the Urinary Tract Infection (UTI) Diagnosis. Electrochemical nitrite sensor was assembled and calibrated, the artificial urine sample was detected; the feasibility of electrochemical nitrite sensor including the errors effect had been checked and proved at around -5.1~2.3%; the possibility to detect artificial UTI urine sample out is around 95.5%; the approximate relationship between the number of E.coli and electrode potential had been built as E=228.3193-3.78225×Ln (N+2.29101e6), thereby building the relationship between UTI possibilities and the measurement. Finally, the conception and design of electrochemical sensor array had been made, thus to measure different biomarkers for the maximum possibilities of UTI and can show the data of the possibility of UTI directly on the screen. Furthermore, it can easily be used and transported for the home-users or patients in hospitals.

[1]  Tao Dong,et al.  A Microfluidic Device for Continuous Sensing of Systemic Acute Toxicants in Drinking Water , 2013, International journal of environmental research and public health.

[2]  A. Ronald The etiology of urinary tract infection: traditional and emerging pathogens. , 2002, The American journal of medicine.

[3]  W. Marsden I and J , 2012 .

[4]  Tao Dong,et al.  A cascade-like silicon filter for improved recovery of oocysts from environmental waters , 2014, Environmental technology.

[5]  M. Finland,et al.  Asymptomatic infections of the urinary tract. , 2002, The Journal of urology.

[6]  Xinyan Zhao,et al.  Multifunctional sample preparation kit and on-chip quantitative nucleic acid sequence-based amplification tests for microbial detection. , 2012, Analytical chemistry.

[7]  T. Dong,et al.  Applied Technology in Diaper-Based UTI Testing for Elder People by Using Nitrite Ion Selective Electrode , 2014 .

[8]  S. Bode-Böger,et al.  Quantification of nitrite and nitrate in human urine and plasma as pentafluorobenzyl derivatives by gas chromatography-mass spectrometry using their 15N-labelled analogs. , 1994, Journal of chromatography. B, Biomedical applications.

[9]  Zhaochu Yang,et al.  Compatible immuno-NASBA LOC device for quantitative detection of waterborne pathogens: design and validation. , 2012, Lab on a chip.

[10]  P. Cochat,et al.  Et al , 2008, Archives de pediatrie : organe officiel de la Societe francaise de pediatrie.

[11]  Tao Dong,et al.  Microfluidic Biosensor Array with Integrated Poly(2,7-Carbazole)/Fullerene-Based Photodiodes for Rapid Multiplexed Detection of Pathogens , 2013, Sensors.

[12]  Nuno M. M. Pires,et al.  Recovery of Cryptosporidium and Giardia organisms from surface water by counter-flow refining microfiltration , 2013, Environmental technology.

[13]  S. Carlsson Antibacterial effects of nitrite in urine , 2005 .

[14]  L. Engstrand,et al.  Effects of pH, nitrite, and ascorbic acid on nonenzymatic nitric oxide generation and bacterial growth in urine. , 2001, Nitric oxide : biology and chemistry.

[15]  L. Engstrand,et al.  Urinary nitrite: more than a marker of infection. , 1997, Urology.

[16]  Carmelo J. Felice,et al.  A pH Sensor Based on a Stainless Steel Electrode Electrodeposited With Iridium Oxide , 2009, IEEE Transactions on Education.