Prevention of Staphylococcus Epidermidis Biofilm Formation Using Electrical Current

A technique for the prevention of staphylococcal adhesion by electrical current exposure was investigated. Teflon coupons were exposed to a continuous flow of 103 cfu/ml Staphylococcus epidermidis with or without 2000 microA DC electrical current delivered by electrodes on opposite sides of a coupon, touching neither each other nor the coupon. A mean 3.46 (SD, 0.20) and 5.70 (SD, 1.03) log10 cfu/cm2 were adhered to the non-electrical current exposed coupons after 4 h and 24 h, respectively. A mean 2.46 (SD, 0.31) and 1.47 (SD, 0.73) log10 cfu/cm2 were adhered after 4 h and 24 h with exposure to 2000 microA electrical current delivered by graphite electrodes. A mean 2.21 (SD, 0.14) and 0.55 (SD, 0.00) log10 cfu/cm2 were adhered after 4 h and 24 h with exposure to 2000 microA electrical current delivered by stainless steel electrodes. Electrical current may be useful in the prevention of staphylococcal adhesion to biomaterials.

[1]  A. D. V. van Staden,et al.  Calcium Orthophosphate-Based Bone Cements (CPCs): Applications, Antibiotic Release and Alternatives to Antibiotics , 2012, Journal of applied biomaterials & functional materials.

[2]  R. Darouiche,et al.  Role of Antibiofilm-Antimicrobial Agents in Controlling Device-Related Infections , 2011, The International journal of artificial organs.

[3]  Carla Renata Arciola,et al.  New Trends in Diagnosis and Control Strategies for Implant Infections , 2011, The International journal of artificial organs.

[4]  Robin Patel,et al.  The Electricidal Effect Is Active in an Experimental Model of Staphylococcus epidermidis Chronic Foreign Body Osteomyelitis , 2009, Antimicrobial Agents and Chemotherapy.

[5]  James M. Steckelberg,et al.  The Electricidal Effect: Reduction of Staphylococcus and Pseudomonas Biofilms by Prolonged Exposure to Low-Intensity Electrical Current , 2008, Antimicrobial Agents and Chemotherapy.

[6]  Robin Patel,et al.  Effect of Electrical Current on the Activities of Antimicrobial Agents against Pseudomonas aeruginosa, Staphylococcus aureus, and Staphylococcus epidermidis Biofilms , 2008, Antimicrobial Agents and Chemotherapy.

[7]  H. C. van der Mei,et al.  Electric block current induced detachment from surgical stainless steel and decreased viability of Staphylococcus epidermidis. , 2005, Biomaterials.

[8]  H. C. van der Mei,et al.  Electric-current-induced detachment of Staphylococcus epidermidis strains from surgical stainless steel. , 2004, Journal of biomedical materials research. Part B, Applied biomaterials.

[9]  H. Busscher,et al.  Controlled electrophoretic deposition of bacteria to surfaces for the design of biofilms. , 2000, Biotechnology and bioengineering.

[10]  H. Harms,et al.  Adhesion of the positively charged bacterium Stenotrophomonas (Xanthomonas) maltophilia 70401 to glass and Teflon , 1996, Journal of bacteriology.

[11]  J. Costerton,et al.  Mechanism of electrical enhancement of efficacy of antibiotics in killing biofilm bacteria , 1994, Antimicrobial Agents and Chemotherapy.

[12]  J. Costerton,et al.  Testing the susceptibility of bacteria in biofilms to antibacterial agents , 1990, Antimicrobial Agents and Chemotherapy.