[Correlation of Epstein-Barr virus-encoded latent membrane protein 1 (LMP1) to fascin and phosphorylated Stat3 in nasopharyngeal carcinoma].

BACKGROUND & OBJECTIVE Latent membrane protein 1 (LMP1) of Epstein-Barr (EB) virus is an important oncogene. Fascin is an actin cross-linking protein involved in cell migration and adhesion. Phosphorylated signal transducer and activator of transcription 3 (pStat3) is a member of STATs family, which is closely related to tumorigenesis. This study was to investigate expressions of LMP1, Fascin and pStat3 in nasopharyngeal carcinoma (NPC) tissues and lymph node metastases of NPC, thus to explore their correlations to the initiation and progression of NPC. METHODS Expressions of EBV-encoded small RNA (EBER), LMP1, Fascin, pStat3, p53, Ki-67 and Bcl-2 were detected in 43 NPC tissues (21 with and 22 without lymph node metastases) and 21 corresponding lymph node metastases using in situ hybridization or immunohistochemistry (IHC). Data were statistically analyzed. Expressions of pStat3 and Fascin were measured in the NPC cell line CNE1 transfected with LMP1-expressing plasmid using Western blot. RESULTS Positive EBER expression was detected in all 43 NPC tissues and 21 lymph node metastases in NPC. The expression rates of LMP1, Fascin, pStat3, p53, Ki-67, and Bcl-2 were 69.8% (30/43), 93.0% (40/43), 72.1% (31/43), 90.7% (39/43), 88.4% (38/43) and 88.4% (38/43)in 43 NPC tissues, respectively. LMP1 expression was positively correlated with the expression level of Fascin, pStat3, p53, Ki-67 and Bcl-2 in 43 NPC cases(P < 0.05). LMP1 was found in 10 out of 21 (46.7%) lymph node metastases in NPC. In addition, LMP1 expression dramatically increased pStat3 and Fascin in CNE1 cells. CONCLUSIONS LMP1 is expressed in lymph node metastatases in NPC. The expression of LMP1 is positively correlated with Fascin, pStat3 and the proliferation index of tumor cells. Moreover, LMP1 up-regulates pStat3 and Fascin in NPC cells.

[1]  M. Ahn,et al.  Phospho‐Stat3 expression and correlation with VEGF, p53, and Bcl‐2 in gastric carcinoma using tissue microarray , 2006, APMIS : acta pathologica, microbiologica, et immunologica Scandinavica.

[2]  N. Dalay,et al.  P53 overexpression in nasopharyngeal carcinoma. , 2004, In Vivo.

[3]  Zhong-ying Shen,et al.  [Expression of fascin 1 gene in the process of the immortalized esophageal carcinoma carcinogenesis]. , 2004, Ai zheng = Aizheng = Chinese journal of cancer.

[4]  J. M. Lee,et al.  The Epstein-Barr virus latency BamHI-Q promoter is positively regulated by STATs and Zta interference with JAK/STAT activation leads to loss of BamHI-Q promoter activity. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[5]  M. Ueffing,et al.  Latent membrane protein 1 of Epstein–Barr virus interacts with JAK3 and activates STAT proteins , 1999, The EMBO journal.

[6]  E. Kieff,et al.  Epstein-Barr virus infection induces expression in B lymphocytes of a novel gene encoding an evolutionarily conserved 55-kilodalton actin-bundling protein , 1994, Journal of virology.

[7]  W. Linehan,et al.  cDNA cloning and expression of the human homolog of the sea urchin fascin and Drosophila singed genes which encodes an actin-bundling protein. , 1994, DNA and cell biology.

[8]  D. Thorley-Lawson,et al.  All three domains of the Epstein-Barr virus-encoded latent membrane protein LMP-1 are required for transformation of rat-1 fibroblasts , 1993, Journal of virology.

[9]  T. Decker,et al.  Cytoplasmic activation of GAF, an IFN‐gamma‐regulated DNA‐binding factor. , 1991, The EMBO journal.

[10]  J. Bryan,et al.  Separation and interaction of the major components of sea urchin actin gel. , 1978, Journal of molecular biology.

[11]  Z. Yong-sheng,et al.  Expression characteristic of STAT3 and its relation to the expression of bcl-2 and LMP1 in nasopharyngeal carcinoma , 2005 .

[12]  G. Xin.  Relationship of EB virus latent membrane protein 1 to matrix metalloproteinase-2 and -9 in nasopharyngeal carcinoma and lymph node metastasis , 2003 .