Quantitative chromatographic methods for the study of enzymic transpeptidation reactions.
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A colorimetric ninhydrin method has been applied to the quantitative estimation of amino acids and peptides on paper chromatograms. This method is suitable for the analysis of enzymic transpeptidation reactions. A procedure for the purification of filter paper for chromatography has been developed.
[1] R. B. Johnston,et al. Elongation of peptide chains in enzyme-catalyzed transamidation reactions. , 1951, Journal of Biological Chemistry.