Pharmacological and toxicity studies of the crude extracts and fractions of Hedranthera barteri leaf in rats and mice

This study assessed the anti-nociceptive and anti-inflammatory potency of the hydro-ethanol extract and fractions from Hedranthera barteri leaf. Column chromatography of the crude extracts, practical acute toxicity testing and fixed dose procedures were carried out. Effects of the extracts on nociception were assessed by tail immersion test in rats, formalin-induced paw licking in rats and acetic acid-induced writhing in mice. The effect of the extracts on inflammation was assessed by carrageenan-induced paw oedema in rats. The hydro-ethanol extracts of Hedranthera barteri reduced nociception and inflammation in a dose-dependent manner. SNG 1, SNG 2 and SNG 3 were separated from the ethanol fractions. The spectra of the compound suggest the presence of a fatty compound, β-sitosterol, as an anti-nociceptive and antiinflammatory compound that may be present in the leaf of the plant. (Afr. J. Biomed. Res. 11: 311 - 321) Key Words: Hedranthera barteri, nociception, inflammation, toxicity, β-sitosterol

[1]  S. Onasanwo,et al.  Anti-nociceptive and anti-inflammatory properties of the leaf extracts of Hedranthera barteri in rats and mice , 2009 .

[2]  J. Staden,et al.  Antibacterial, anti-inflammatory and antimalarial activities of some Nigerian medicinal plants , 2005 .

[3]  P. Botham,et al.  Acute systemic toxicity--prospects for tiered testing strategies. , 2004, Toxicology in vitro : an international journal published in association with BIBRA.

[4]  R. Yunes,et al.  In vitro effect of the extract and the 1,7-dihydroxy-2,3-dimethoxy xanthone from Polygala cyparissias on the contractions induced by inflammatory mediators and ovalbumin in normal and actively sensitised trachea from guinea pig , 1999, Inflammation Research.

[5]  P. Uma Devi,et al.  In vivo radioprotection by ocimum flavonoids: survival of mice. , 1999, Radiation research.

[6]  Y. Öztürk,et al.  Nepetalactone: A New Opioid Analgesic from Nepeta caesarea Boiss , 1998, The Journal of pharmacy and pharmacology.

[7]  R. Yunes,et al.  Three xanthones from Polygala cyparissias , 1998 .

[8]  Kjell Hole,et al.  The formalin test in mice: dissociation between inflammatory and non-inflammatory pain , 1987, Pain.

[9]  A. Bauer,et al.  Antibiotic susceptibility testing by a standardized single disk method. , 1966, American journal of clinical pathology.

[10]  C A WINTER,et al.  Carrageenin-Induced Edema in Hind Paw of the Rat as an Assay for Antiinflammatory Drugs , 1962, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[11]  R. Koster,et al.  ACETIC ACID FOR ANALGESIC SCREENING , 1959 .

[12]  R. Cadmus,et al.  A Method for Evaluating both Non-Narcotic and Narcotic Analgesics , 1957, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[13]  J. Dalziel,et al.  The useful plants of West Tropical Africa. , 1938 .