Circulating concentrations of dimeric inhibin A and B in the male rhesus monkey (Macaca mulatta).

The purpose of this study was to determine the relative concentrations of inhibin A and B in peripheral serum of the adult male rhesus monkey and to examine the testicular contribution to these circulating forms of inhibin. In addition, inhibin B concentrations were also determined in peripheral sera of neonatal and juvenile males and in spermatic vein blood of adults. Immunoradiometric assays specific for the measurement of inhibin A and B were used. These assays also provided an opportunity to reexamine the physiological significance of a replacement infusion of recombinant human (rh)-inhibin A previously employed to study the role of this hormone in regulating FSH secretion in the monkey. In intact adults, the mean (+/-SE) serum concentration of inhibin B was 1008 +/- 184 pg/mL. In contrast, circulating inhibin A concentrations were very low (< 46 pg/mL). Inhibin B was consistently detected in neonatal monkey serum (275 +/- 57 pg/mL), and concentrations of this inhibin dimer increased throughout postnatal development, reaching maximum values in adulthood. Circulating inhibin A concentrations in neonatal and juvenile monkeys were undetectable (< 7 pg/mL). Both forms of inhibin were generally undetectable in castrate sera. The ratio of inhibin B concentrations in testicular venous blood to those in the peripheral circulation was 1.4:1. These findings indicate that, in the male monkey, inhibin B is the principal form of circulating dimeric inhibin, and that this hormone is derived exclusively from the testis. The elevated levels of circulating inhibin B in the juvenile male monkey suggest that, during this phase of development, testicular inhibin B secretion is relatively gonadotropin independent. Additionally, we found that the concentration of circulating inhibin A in castrate animals that had earlier received an iv infusion of rh-inhibin A (832 ng/h/kg BW) was 9881 +/- 2135 pg/mL, indicating that this mode of inhibin replacement may not have been entirely physiological.

[1]  W. Crowley,et al.  Inhibin B secretion in males with gonadotropin-releasing hormone (GnRH) deficiency before and during long-term GnRH replacement: relationship to spontaneous puberty, testicular volume, and prior treatment--a clinical research center study. , 1996, The Journal of clinical endocrinology and metabolism.

[2]  A. Mcneilly,et al.  Serum inhibin B levels reflect Sertoli cell function in normal men and men with testicular dysfunction. , 1996, The Journal of clinical endocrinology and metabolism.

[3]  A. R. Midgley,et al.  Development of a two-site solid-phase immunochemiluminescent assay for measurement of dimeric inhibin-A in human serum and other biological fluids. , 1996, Clinical chemistry.

[4]  T. Plant,et al.  Puberty occurring either spontaneously or induced precociously in rhesus monkey (Macaca mulatta) is associated with a marked proliferation of Sertoli cells. , 1996, Biology of reproduction.

[5]  A. Mcneilly,et al.  Measurement of dimeric inhibin B throughout the human menstrual cycle. , 1996, The Journal of clinical endocrinology and metabolism.

[6]  A. Mcneilly,et al.  Inhibin-B: a likely candidate for the physiologically important form of inhibin in men. , 1996, The Journal of clinical endocrinology and metabolism.

[7]  S. Majumdar,et al.  Replacement with recombinant human inhibin immediately after orchidectomy in the hypophysiotropically clamped male rhesus monkey (Macaca mulatta) maintains follicle-stimulating hormone (FSH) secretion and FSH beta messenger ribonucleic acid levels at precastration values. , 1995, Endocrinology.

[8]  D. Baird,et al.  Detection of dimeric inhibin throughout the human menstrual cycle by two‐site enzyme immunoassay , 1994, Clinical endocrinology.

[9]  S. Sakoda,et al.  Hormonal Regulation of Human Testicular Inhibin α and βB Subunit Messenger Rnas , 1993 .

[10]  R. Medhamurthy,et al.  A comparison of moment to moment and diurnal changes in circulating inhibin and testosterone concentrations in male rhesus monkeys (Macaca mulatta). , 1991, Endocrinology.

[11]  N. Groome,et al.  Preparation of monoclonal antibodies to the beta A subunit of ovarian inhibin using a synthetic peptide immunogen. , 1991, Hybridoma.

[12]  R. Sharpe,et al.  Regulation of Sertoli cell inhibin production and of inhibin α-subunit mRNA levels by specific germ cell types , 1990, Molecular and Cellular Endocrinology.

[13]  S. Winters,et al.  Developmental changes in testicular inhibin and androgen-binding protein during sexual maturation in the cynomolgus monkey, Macaca fascicularis. , 1990, Endocrinology.

[14]  R. Medhamurthy,et al.  Immunoneutralization of circulating inhibin in the hypophysiotropically clamped male rhesus monkey (Macaca mulatta) results in a selective hypersecretion of follicle-stimulating hormone. , 1990, Endocrinology.

[15]  K. Fukutani,et al.  Measurement of inhibin concentrations in men: study of changes after castration and comparison with androgen levels in testicular tissue, spermatic venous blood, and peripheral venous blood. , 1990, The Journal of clinical endocrinology and metabolism.

[16]  A. Grootenhuis,et al.  Inhibin in immature rat Sertoli cell conditioned medium: A 32 kDa αβ-B dimer , 1990, Molecular and Cellular Endocrinology.

[17]  N. Groome,et al.  Monoclonal and polyclonal antibodies reactive with the 1-32 amino terminal sequence of the alpha subunit of human 32K inhibin. , 1990, Hybridoma.

[18]  S. Winters.  Inhibin is released together with testosterone by the human testis. , 1990, The Journal of clinical endocrinology and metabolism.

[19]  P. Sawchenko,et al.  Differential production and regulation of inhibin subunits in rat testicular cell types. , 1989, Endocrinology.

[20]  E. Nieschlag,et al.  Radioimmunoassay of inhibin in the serum of male monkeys. , 1989, The Journal of endocrinology.

[21]  W. Vale,et al.  Circulating inhibin alpha concentrations in infant, prepubertal, and adult male rhesus monkeys (Macaca mulatta) and in juvenile males during premature initiation of puberty with pulsatile gonadotropin-releasing hormone treatment. , 1989, Endocrinology.

[22]  G. Risbridger,et al.  In vivo and in vitro production of inhibin by cryptorchid testes from adult rats. , 1989, Endocrinology.

[23]  A. Zeleznik,et al.  In the rhesus monkey (Macaca mulatta), the negative feedback regulation of follicle-stimulating hormone secretion by an action of testicular hormone directly at the level of the anterior pituitary gland cannot be accounted for by either testosterone or estradiol. , 1987, Endocrinology.

[24]  S. Winters,et al.  Identification of inhibin secreted by cynomolgus monkey Sertoli cell cultures. , 1987, The Journal of clinical endocrinology and metabolism.

[25]  T. Plant.  A study of the role of the postnatal testes in determining the ontogeny of gonadotropin secretion in the male rhesus monkey (Macaca mulatta). , 1985, Endocrinology.

[26]  T. Plant,et al.  Evidence from the rhesus monkey (Macaca mulatta) for the view that negative feedback control of luteinizing hormone secretion by the testis is mediated by a deceleration of hypothalamic gonadotropin-releasing hormone pulse frequency. , 1984, Endocrinology.

[27]  D. Hess,et al.  Testosterone and the control of gonadotropin secretion in the male rhesus monkey (Macaca mulatta). , 1978, Endocrinology.

[28]  D. R. Mccullagh.  DUAL ENDOCRINE ACTIVITY OF THE TESTES. , 1932, Science.

[29]  W. Vale,et al.  Gonadal and extragonadal expression of inhibin alpha, beta A, and beta B subunits in various tissues predicts diverse functions. , 1988, Proceedings of the National Academy of Sciences of the United States of America.