Effect of Growth Stage and Solvent Extract on the Antioxidant Potential of Olive Leaves

The effect of growth stage on the physicochemical composition and antioxydant activities of olive leaves extracted with solvents of increasing polarity was investigated at two different stages. The total phenolic, total o-diphenols and total flavonoid were measured. Therefore, the levels of chlorophyll and carotenoids were also measured. The content of phenols varied significantly with two different growth stages October and February. The lowest level of phenols and o-diphenols was detected in the first vegetative stage whereas the highest content was enregistered during the second vegetative stage. The results showed that methanol extract contains the maximum phenolic content. Furthermore, the differences in total flavonoid contents of leaves between two different stages and between solvent extract were highly significant. Thus, the AA exhibited asignificant (p<0.05) increase during leaves developpement. In fact, methanol and ethyl acetate extract was a considerably more effective on DPPH radical scavenger than other solvents extracts.

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