论文信息 - Estimating Oligo-nucleotide Microarray Expression by Hybridization Process Modelling

Estimating Oligo-nucleotide Microarray Expression by Hybridization Process Modelling

Oligonucleotide microarray technology has become widely spread in genetic probing for different purposes. This technology has shown to be highly reliable and dependable, although not free from problems regarding expression estimation, as it relies on the differential or contrasted hybridization of test probes. In many cases hybridization results do not show the expected behaviour, thus affecting to the reliability of expression estimation. In the present paper a modelling of the hybridization process is proposed which can be used to predict and correct expression levels on a specific test probe, improving the reliability of the results. Some examples from real microarray processing are shown and discussed

[1] Wei-Min Liu,et al. Robust estimators for expression analysis , 2002, Bioinform..

[2] Susan R. Wilson,et al. Statistical Analysis of Adsorption Models for Oligonucleotide Microarrays , 2004, Statistical applications in genetics and molecular biology.

[3] María Victoria Rodellar Biarge,et al. Robust Preprocessing of Gene Expression Microarrays for Independent Component Analysis , 2006, ICA.

[4] Mark Pollitt,et al. Exploration , 2006, J. Digit. Forensic Pract..

[5] Rafael A Irizarry,et al. Exploration, normalization, and summaries of high density oligonucleotide array probe level data. , 2003, Biostatistics.

[6] A. Venetsanopoulos,et al. A multichannel order-statistic technique for cDNA microarray image processing , 2004, IEEE Transactions on NanoBioscience.

[7] C. Li,et al. Model-based analysis of oligonucleotide arrays: expression index computation and outlier detection. , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[8] Linda R. Petzold,et al. Stochastic modelling of gene regulatory networks , 2005 .

[9] S. P. Fodor,et al. High density synthetic oligonucleotide arrays , 1999, Nature Genetics.

[10] R. Stoughton,et al. Use of hybridization kinetics for differentiating specific from non-specific binding to oligonucleotide microarrays. , 2002, Nucleic acids research.