Sequential Shift Absolute Phase Aberration Calibration in Digital Holographic Phase Imaging Based on Chebyshev Polynomials Fitting

We propose a novel absolute calibrate method for digital holographic microscopy with the sequential shift method using Chebyshev polynomials. We separate the object phase and the aberrations by sequential shifting the sample twice in vertical plane of the optical axis. The aberrations phase is then calculated using the high order Chebyshev polynomials. The correct phase is obtained by subtracting the aberrations from the original phase containing the aberration. This method can compensate for the complex aberrations including high-order aberrations without changing the traditional optical system. Meanwhile, it can effectively protect the medium and high frequency information of the specimen in the phase image. Numerical simulation and experimental results demonstrate the availability and advantages of the absolute calibrate method.

[1]  Zhaojun Liu,et al.  Three-step shift-rotation absolute measurement of optical surface figure with irregular shaped aperture , 2018, Optics Communications.

[2]  M. Atlan,et al.  Imaging gold nanoparticles in living cell environments using heterodyne digital holographic microscopy. , 2009, Optics express.

[3]  Jiantai Dou,et al.  Robust phase aberration compensation in digital holographic microscopy by self-extension of holograms , 2019, Optics Communications.

[4]  Carlos Trujillo,et al.  Automatic full compensation of quantitative phase imaging in off-axis digital holographic microscopy. , 2016, Applied optics.

[5]  Ning Xi,et al.  Investigating dynamic structural and mechanical changes of neuroblastoma cells associated with glutamate-mediated neurodegeneration , 2014, Scientific Reports.

[6]  Q. Yuan,et al.  Generalized shift-rotation absolute measurement method for high-numerical-aperture spherical surfaces with global optimized wavefront reconstruction algorithm. , 2017, Optics express.

[7]  P. Ferraro,et al.  Direct full compensation of the aberrations in quantitative phase microscopy of thin objects by a single digital hologram , 2007 .

[8]  Jianlin Zhao,et al.  Phase aberration compensation of digital holographic microscopy based on least squares surface fitting , 2009 .

[9]  Qiusheng Lian,et al.  Automatic phase aberration compensation for digital holographic microscopy based on phase variation minimization. , 2018, Optics letters.

[11]  Van Lam,et al.  Automatic phase aberration compensation for digital holographic microscopy based on deep learning background detection. , 2017, Optics express.

[12]  E. Bloemhof,et al.  Absolute surface metrology by differencing spatially shifted maps from a phase-shifting interferometer. , 2010, Optics letters.

[13]  Mikael Sjödahl,et al.  Improved particle position accuracy from off-axis holograms using a Chebyshev model. , 2018, Applied optics.

[14]  J C Wyant,et al.  Testing spherical surfaces: a fast, quasi-absolute technique. , 1992, Applied optics.

[15]  Yongxin Sui,et al.  Absolute surface figure testing by shift-rotation method using Zernike polynomials. , 2012, Optics letters.

[17]  Baoli Yao,et al.  Simple and fast spectral domain algorithm for quantitative phase imaging of living cells with digital holographic microscopy , 2017, BiOS.

[18]  Jiubin Tan,et al.  Absolute spherical surface metrology by differencing rotation maps. , 2015, Applied optics.

[19]  E. Cuche,et al.  Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms. , 1999, Applied optics.

[20]  Renu John,et al.  Optofluidic bioimaging platform for quantitative phase imaging of lab on a chip devices using digital holographic microscopy. , 2016, Applied optics.

[21]  Yu Wu,et al.  Simple and flexible phase compensation for digital holographic microscopy with electrically tunable lens. , 2017, Applied optics.

[22]  Pietro Ferraro,et al.  Compensation of the inherent wave front curvature in digital holographic coherent microscopy for quantitative phase-contrast imaging. , 2003, Applied optics.

[23]  Qian Chen,et al.  Optimal principal component analysis-based numerical phase aberration compensation method for digital holography. , 2016, Optics letters.

[24]  W. Song,et al.  Simple and rapid data-reduction method with pixel-level spatial frequency of shift-rotation method. , 2013, Applied optics.

[25]  Qian Chen,et al.  Phase aberration compensation in digital holographic microscopy based on principal component analysis. , 2013, Optics letters.

[26]  Christian Depeursinge,et al.  Cell morphology and intracellular ionic homeostasis explored with a multimodal approach combining epifluorescence and digital holographic microscopy , 2010, Journal of biophotonics.

[27]  Christian Depeursinge,et al.  Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy. , 2009, Journal of biomedical optics.

[28]  Johannes A. Soons,et al.  A simple ball averager for reference sphere calibrations , 2005, SPIE Optics + Photonics.