In vitro measurements of physiological glucose concentrations in biological fluids using mid-infrared light

Mid-infrared transmission spectroscopy using broadband mid-infrared or Quantum Cascade laser sources is used to predict glucose concentrations of aqueous and serum solutions containing physiologically relevant amounts of glucose (50-400 mg/dL). We employ partial least squares regression to generate a calibration model using a subset of the spectra taken and to predict concentrations from new spectra. Clinically accurate measurements with respect to a Clarke error grid were made for concentrations as low as 30 mg/dL, regardless of background solvent. These results are an important and encouraging step in the work towards developing a noninvasive in vivo glucose sensor in the mid-infrared.

[1]  Andreas Mandelis,et al.  Noninvasive glucose detection in human skin using wavelength modulated differential laser photothermal radiometry , 2012, Biomedical optics express.

[2]  O. Khalil,et al.  Spectroscopic and clinical aspects of noninvasive glucose measurements. , 1999, Clinical chemistry.

[3]  D. Cox,et al.  Evaluating Clinical Accuracy of Systems for Self-Monitoring of Blood Glucose , 1987, Diabetes Care.

[4]  Y. Ozaki,et al.  In Vivo Noninvasive Measurement of Blood Glucose by Near-Infrared Diffuse-Reflectance Spectroscopy , 2003, Applied spectroscopy.

[5]  S. Mirov,et al.  Middle Infrared, Quantum Cascade Laser Optoelectronic Absorption System for Monitoring Glucose in Serum , 2005, Applied spectroscopy.

[6]  Adrian Mariampillai,et al.  Quantification of glucose levels in flowing blood using M-mode swept source optical coherence tomography , 2012 .

[7]  Bernhard Lendl,et al.  Direct determination of glucose, lactate and triglycerides in blood serum by a tunable quantum cascade laser-based mid-IR sensor , 2013 .

[8]  Valery V. Tuchin,et al.  Handbook of Optical Sensing of Glucose in Biological Fluids and Tissues , 2008 .

[9]  G. B. Christison,et al.  Laser photoacoustic determination of physiological glucose concentrations in human whole blood , 1993, Medical and Biological Engineering and Computing.

[10]  A. Michel,et al.  In vivo measurement of mid-infrared light scattering from human skin , 2013, Biomedical optics express.

[11]  S. D. Jong.  SIMPLS: an alternative approach to partial least squares regression , 1993 .

[12]  Mid-infrared (λ = 8.4–9.9 μm) light scattering from porcine tissue , 2012 .

[13]  R. Marbach.  A New Method for Multivariate Calibration , 2005 .

[14]  R. Dasari,et al.  Investigation of the specificity of Raman spectroscopy in non-invasive blood glucose measurements , 2011, Analytical and bioanalytical chemistry.

[15]  Yargo Bonetti,et al.  External cavity quantum cascade laser tunable from 7.6 to 11.4 μm , 2009 .

[16]  Werner Mäntele,et al.  A novel approach to non-invasive glucose measurement by mid-infrared spectroscopy: The combination of quantum cascade lasers (QCL) and photoacoustic detection , 2005 .