Generating extended images of the corneal nerve plexus by guided eye movements

Confocal laser scanning microscopy (CLSM) provides a technique for in vivo imaging the sub-basal nerve plexus (SNP) of the cornea. For mapping an extended area of the SNP, currently the objective of the CLSM is manually moved laterally across the corneal surface during acquisition of overlapping images. This work presents a proof of concept for a new method that realizes the movements during the mapping process by guiding the eye movements with a computercontrolled fixation target on a display in front of the contralateral eye. The purpose is to generate a maximized and completely covered scan area with an overlapping area of adjacent images of more than 50%.