Comparison of Microscale Ultracentrifugation and Equilibrium Dialysis for the Determination of the Unbound Fraction of Theophylline in Human Serum

A microscale ultracentrifugation procedure for the measurement of free theophylline in serum is described and evaluated by comparison with an equilibrium dialysis method. Provided an ultracentrifuge is available, micro-ultracentrifugation is a simple, cost-effective technique for the routine determination of free drug fractions.

[1]  I. Watson,et al.  Micro-Scale Ultracentrifugation as an Alternative to Ultrafiltration for the Determination of the Unbound Fraction of Phenytoin in Human Serum , 1986, Annals of clinical biochemistry.

[2]  M. Woodruff,et al.  Free drug concentration monitoring in clinical practice. Rationale and current status. , 1986, Clinical pharmacokinetics.

[3]  D. Buss,et al.  The ex vivo plasma protein binding of theophylline in renal disease. , 1985, British journal of clinical pharmacology.

[4]  D. Buss,et al.  Protein binding of theophylline. , 1985, British journal of clinical pharmacology.

[5]  D. Buss,et al.  Free and total plasma theophylline concentrations in chronic airflow obstruction. , 1984, Thorax.

[6]  C. Knott,et al.  Do saliva concentrations predict plasma unbound theophylline concentrations? A problem re-examined. , 1984, British journal of clinical pharmacology.

[7]  U. Gundert-Remy,et al.  Binding of theophylline and its metabolites to human plasma proteins. , 1983, British journal of clinical pharmacology.

[8]  D. Sandnes,et al.  Binding of theophylline in human serum determined by ultrafiltration and equilibrium dialysis. , 1983, British journal of clinical pharmacology.

[9]  D. Buss,et al.  Determinants of the plasma protein binding of theophylline in health. , 1983, British journal of clinical pharmacology.

[10]  L. Fields,et al.  Factors influencing theophylline serum protein binding , 1982, Clinical pharmacology and therapeutics.

[11]  J. Mucklow.  The Use of Saliva in Therapeutic Drug Monitoring , 1982 .

[12]  Ogilvie Ri.  Monitoring plasma theophylline concentrations. , 1980 .