论文信息 - Establishment of multiple shoot clumps from maize (Zea mays L.) and regeneration of herbicide-resistant transgenic plantlets

Establishment of multiple shoot clumps from maize (Zea mays L.) and regeneration of herbicide-resistant transgenic plantlets

A kind of quick, efficient and season-free inducing embryoid and multiple shoot clumps system from shoot tip meristems that derived from elite inbreds of maize was established. The herbicide-resistant gene als (coding Acetolactate synthase) isolated from a mutant of Arabidopsis thaliana was transferred to tissue pieces of maize multiple shoot clumps by microprojectile bombardment. Herbicide-resistant tissue and regenerants were obtained through selections with herbicide chlorsulfuron. PCR analysis and Southern blot hybridization indicated that gene als has been transferred to some regenerants. The test of spraying chlorsulfuron displayed that the transgenic plantlets and R1 plants had favorable herbicide-resistant trait. We have established a new genotype-free system of maize which could rapidly and efficiently produce large quantities of transgenic plantlets.

[1] B. Gordon-Kamm,et al. Germline Transformation of Maize Following Manipulation of Chimeric Shoot Meristems , 1995, Bio/Technology.

[2] P. Lemaux,et al. Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants. , 1990, The Plant cell.

[3] D. Becker,et al. Efficient transformation of scutellar tissue of immature maize embryos , 1997, Theoretical and Applied Genetics.

[4] L. Crossland,et al. Field Performance of Elite Transgenic Maize Plants Expressing an Insecticidal Protein Derived from Bacillus thuringiensis , 1993, Bio/Technology.

[5] M. Fromm,et al. Inheritance and Expression of Chimeric Genes in the Progeny of Transgenic Maize Plants , 1990, Bio/Technology.

[6] J. Blanco,et al. Methods of obtaining maize totipotent tissues. I. Seedling segments culture , 1984 .

[7] C. Srinivasan,et al. In-vitro morphogenesis of corn (Zea mays L.) , 1992, Planta.

[8] J. Dai,et al. [Studies of transgene segregation and integration in maize]. , 1999, Yi chuan xue bao = Acta genetica Sinica.

[9] J. Widholm,et al. Type I callus as a bombardment target for generating fertile transgenic maize (Zea mays L.) , 1995, Planta.

[10] R. Lundquist,et al. Transformation and inheritance of a hygromycin phosphotransferase gene in maize plants , 2004, Plant Molecular Biology.

[11] Juren Zhang,et al. Direct differentiation of ears and tassels from cultured shoot apices of maize , 1999, Science in China Series C: Life Sciences.