Efficient genetic engineering of human intestinal organoids using electroporation
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Toshiro Sato | M. Matano | K. Nanki | Masayuki Fujii | Mami Matano | Toshiro Sato | Kosaku Nanki | Masayuki Fujii
[1] C. Sorg,et al. Electroporation efficiency in mammalian cells is increased by dimethyl sulfoxide (DMSO). , 1996, Nucleic acids research.
[2] Hans Clevers,et al. Functional repair of CFTR by CRISPR/Cas9 in intestinal stem cell organoids of cystic fibrosis patients. , 2013, Cell stem cell.
[3] Min Han,et al. Efficient Transposition of the piggyBac (PB) Transposon in Mammalian Cells and Mice , 2005, Cell.
[4] Takanori Kanai,et al. Modeling colorectal cancer using CRISPR-Cas9–mediated engineering of human intestinal organoids , 2015, Nature Medicine.
[5] H. Clevers,et al. Single Lgr5 stem cells build cryptvillus structures in vitro without a mesenchymal niche , 2009, Nature.
[6] H. Clevers,et al. Controlled gene expression in primary Lgr5 organoid cultures , 2011, Nature Methods.
[7] H. Clevers,et al. Generation of BAC Transgenic Epithelial Organoids , 2013, PloS one.
[8] David A. Scott,et al. Genome engineering using the CRISPR-Cas9 system , 2013, Nature Protocols.
[9] Hans Clevers,et al. Sequential cancer mutations in cultured human intestinal stem cells , 2015, Nature.
[10] Hans Clevers,et al. Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett's epithelium. , 2011, Gastroenterology.
[11] Matthias Stelzner,et al. Isolation and characterization of intestinal stem cells based on surface marker combinations and colony-formation assay. , 2013, Gastroenterology.