X-ray phase contrast for CO2 microangiography

We demonstrate a laboratory method for imaging small blood vessels using x-ray propagation-based phase-contrast imaging and carbon dioxide (CO(2)) gas as a contrast agent. The limited radiation dose in combination with CO(2) being clinically acceptable makes the method promising for small-diameter vascular visualization. We investigate the possibilities and limitations of the method for small-animal angiography and compare it with conventional absorption-based x-ray angiography. Photon noise in absorption-contrast imaging prevents visualization of blood vessels narrower than 50 µm at the highest radiation doses compatible with living animals, whereas our simulations and experiments indicate the possibility of visualizing 20 µm vessels at radiation doses as low as 100 mGy. Experimental computed tomography of excised rat kidney shows blood vessels of diameters down to 60 µm with improved image quality compared to absorption-based methods. With our present prototype x-ray source, the acquisition time for a tomographic dataset is approximately 1 h, which is long compared to the 1-20 min common for absorption-contrast micro-CT systems. Further development of the liquid-metal-jet microfocus x-ray sources used here and high-resolution x-ray detectors shows promise to reduce exposure times and make this high-resolution method practical for imaging of living animals.

[1]  V. Cnudde,et al.  Software tools for quantification of X-ray microtomography at the UGCT , 2007 .

[2]  Irvin F. Hawkins,et al.  Carbon Dioxide Angiography: Principles, Techniques, and Practices , 2007 .

[3]  Richard J. Fitzgerald,et al.  Phase‐Sensitive X‐Ray Imaging , 2000 .

[4]  A. Momose.  Recent Advances in X-ray Phase Imaging , 2005 .

[5]  J. Goodman.  Introduction to Fourier optics , 1969 .

[6]  S. Wilkins,et al.  Phase-contrast imaging using polychromatic hard X-rays , 1996, Nature.

[7]  T Takeda,et al.  Blood vessels: depiction at phase-contrast X-ray imaging without contrast agents in the mouse and rat-feasibility study. , 2000, Radiology.

[8]  K. Hanson,et al.  Detectability in computed tomographic images. , 1979, Medical physics.

[9]  Jan Grimm,et al.  An X-ray computed tomography imaging agent based on long-circulating bismuth sulphide nanoparticles , 2006, Nature materials.

[10]  E. Kvašňák,et al.  Temperature dependence of blood surface tension. , 2007, Physiological research.

[11]  M. Anastasio,et al.  Propagation based differential phase contrast imaging and tomography of murine tissue with a laser plasma x-ray source , 2007 .

[12]  A. Rose.  The sensitivity performance of the human eye on an absolute scale. , 1948, Journal of the Optical Society of America.

[13]  Wolfhard Semmler,et al.  Volumetric computed tomography (VCT): a new technology for noninvasive, high-resolution monitoring of tumor angiogenesis , 2004, Nature Medicine.

[14]  Lynn Kissel,et al.  RTAB: the Rayleigh scattering database , 2000 .

[15]  D. Mattson,et al.  Comparison of arterial blood pressure in different strains of mice. , 2001, American journal of hypertension.

[16]  Anna Burvall,et al.  Phase Retrieval in X-ray Phase-contrast Imaging Suitable for Tomography , 2022 .

[17]  G. Johnson,et al.  In vivo small-animal imaging using micro-CT and digital subtraction angiography , 2008, Physics in medicine and biology.

[18]  Hans M. Hertz,et al.  Liquid-metal-jet anode electron-impact x-ray source , 2003 .

[19]  J. Linnros,et al.  Performance of an X-ray imaging detector based on a structured scintillator , 2009 .

[20]  K K W Siu,et al.  Dynamic imaging of the lungs using x-ray phase contrast , 2005, Physics in medicine and biology.

[21]  T Aakhus,et al.  [Digital subtraction angiography]. , 1983, Tidsskrift for den Norske laegeforening : tidsskrift for praktisk medicin, ny raekke.

[22]  Atsushi Momose,et al.  Phase–contrast X–ray computed tomography for observing biological soft tissues , 1996, Nature Medicine.

[23]  O. Bunk,et al.  Phase retrieval and differential phase-contrast imaging with low-brilliance X-ray sources , 2006 .

[24]  B. Funaki.  Carbon dioxide angiography. , 2008, Seminars in interventional radiology.

[25]  G Allan Johnson,et al.  Tumor imaging in small animals with a combined micro-CT/micro-DSA system using iodinated conventional and blood pool contrast agents. , 2006, Contrast media & molecular imaging.

[26]  W. Boron,et al.  Medical physiology : a cellular and molecular approach , 2002 .

[27]  Q. Yuan,et al.  Mouse blood vessel imaging by in-line x-ray phase-contrast imaging , 2008, Physics in medicine and biology.

[28]  Nobunao Maehara,et al.  Experimental microcomputed tomography study of the 3D microangioarchitecture of tumors , 2003, European Radiology.

[29]  Christoph Groden,et al.  Vascular imaging in small rodents using micro-CT. , 2010, Methods.

[30]  S. Wilkins,et al.  Phase-contrast imaging of weakly absorbing materials using hard X-rays , 1995, Nature.

[31]  T E Gureyev,et al.  Quantitative X-ray projection microscopy: phase-contrast and multi-spectral imaging. , 2002, Journal of microscopy.

[32]  S. Wilkins,et al.  Simultaneous phase and amplitude extraction from a single defocused image of a homogeneous object , 2002, Journal of microscopy.

[33]  Zoltan Derdak,et al.  Low density contrast agents for x-ray phase contrast imaging: the use of ambient air for x-ray angiography of excised murine liver tissue , 2008, Physics in medicine and biology.

[34]  P. Tarazona.  Capillarity and Wetting , 1987 .

[35]  G Margaritondo,et al.  Synchrotron microangiography with no contrast agent , 2004, Physics in medicine and biology.

[36]  Kentaro Uesugi,et al.  IMAGING LUNG AERATION AND LUNG LIQUID CLEARANCE AT BIRTH USING PHASE CONTRAST X‐RAY IMAGING , 2009, Clinical and experimental pharmacology & physiology.

[37]  G Allan Johnson,et al.  4-D Micro-CT of the Mouse Heart , 2005, Molecular imaging.

[38]  R. Lewis,et al.  Medical phase contrast x-ray imaging: current status and future prospects. , 2004, Physics in medicine and biology.

[39]  R. Cerbino.  Capillarity and Wetting Phenomena: Drops, Bubbles, Pearls, Waves , 2006 .

[40]  Hans M. Hertz,et al.  Phase-contrast x-ray imaging with a liquid-metal-jet-anode microfocus source , 2007 .

[41]  L. Hedlund,et al.  A liposomal nanoscale contrast agent for preclinical CT in mice. , 2006, AJR. American journal of roentgenology.

[42]  G Allan Johnson,et al.  Effects of breathing and cardiac motion on spatial resolution in the microscopic imaging of rodents , 2005, Magnetic resonance in medicine.

[43]  Paola Coan,et al.  In vivo x-ray phase contrast analyzer-based imaging for longitudinal osteoarthritis studies in guinea pigs , 2010, Physics in medicine and biology.

[44]  G. Nikiforidis,et al.  In vivo small animal imaging: current status and future prospects. , 2010, Medical physics.

[45]  H. Hertz,et al.  Liquid-metal-jet anode x-ray tube , 2004 .

[46]  James O. Berger.  Statistical Decision Theory , 1980 .

[47]  J. H. Hubbell,et al.  Tables of X-Ray Mass Attenuation Coefficients and Mass Energy-Absorption Coefficients 1 keV to 20 MeV for Elements Z = 1 to 92 and 48 Additional Substances of Dosimetric Interest , 1995 .