Peptide‐Grafted Nanodiamonds: Preparation, Cytotoxicity and Uptake in Cells

Nanodiamonds that were prepared by high pressure/high temperature were functionalised with biomolecules for biological applications. Nanodiamonds (NDs, ≤35 nm) that were coated by silanisation or with polyelectrolyte layers were grafted with a fluorescent thiolated peptide via a maleimido function; this led to an aqueous colloidal suspension that was stable for months. These substituted NDs were not cytotoxic for CHO cells. Their capacity to enter mammalian cells, and their localisation inside were ascertained after labelling the nucleus and actin, by examining the cells by confocal, reflected light and fluorescence microscopy.

[1]  K. Kneipp,et al.  Surface-enhanced Raman scattering in local optical fields of silver and gold nanoaggregates-from single-molecule Raman spectroscopy to ultrasensitive probing in live cells. , 2006, Accounts of chemical research.

[2]  S. Xiao,et al.  Surface reactions of 4-aminothiophenol with heterobifunctional crosslinkers bearing both succinimidyl ester and maleimide for biomolecular immobilization. , 2005, Journal of colloid and interface science.

[3]  Saber M Hussain,et al.  Are diamond nanoparticles cytotoxic? , 2007, The journal of physical chemistry. B.

[4]  Jun Lin,et al.  Synthesis and characterization of urea bridged hybrid periodic mesoporous organosilica materials , 2008 .

[5]  P. Alivisatos.  The use of nanocrystals in biological detection , 2004, Nature Biotechnology.

[6]  F. Miranda,et al.  Surface analysis monitoring of polyelectrolyte deposition on Ba0.5Sr0.5TiO3 thin films , 2007 .

[7]  S. Sagan,et al.  Tracking a new cell-penetrating (W/R) nonapeptide, through an enzyme-stable mass spectrometry reporter tag. , 2007, Analytical chemistry.

[8]  K. Holt.  Diamond at the nanoscale: applications of diamond nanoparticles from cellular biomarkers to quantum computing , 2007, Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences.

[9]  E. Kaiser,et al.  Color test for detection of free terminal amino groups in the solid-phase synthesis of peptides. , 1970, Analytical biochemistry.

[10]  Erik Pierstorff,et al.  Active nanodiamond hydrogels for chemotherapeutic delivery. , 2007, Nano letters.

[11]  Huan-Cheng Chang,et al.  Adsorption and immobilization of cytochrome c on nanodiamonds. , 2004, Langmuir : the ACS journal of surfaces and colloids.

[12]  Elena Perevedentseva,et al.  Direct and in vitro observation of growth hormone receptor molecules in A549 human lung epithelial cells by nanodiamond labeling , 2007 .

[13]  N. Kaji,et al.  Single-molecule measurements with a single quantum dot. , 2007, Chemical record.

[14]  Elena Perevedentseva,et al.  Spectroscopic study of bio-functionalized nanodiamonds , 2006 .

[15]  M. Prato,et al.  Functionalized carbon nanotubes in drug design and discovery. , 2008, Accounts of chemical research.

[16]  Chia-Liang Cheng,et al.  The interaction of the protein lysozyme with bacteria E. coli observed using nanodiamond labelling , 2007 .

[17]  Anke Krüger,et al.  Surface functionalisation of detonation diamond suitable for biological applications , 2006 .

[18]  S. Parker.  Vibrational spectroscopy of N-phenylmaleimide. , 1995, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[19]  A. Krueger,et al.  New carbon materials: biological applications of functionalized nanodiamond materials. , 2008, Chemistry.

[20]  S. Maiti,et al.  Molecular Effects of Uptake of Gold Nanoparticles in HeLa Cells , 2007, Chembiochem : a European journal of chemical biology.

[21]  Erkki Ruoslahti,et al.  Nanocrystal targeting in vivo , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[22]  Raphaël Lévy.  Peptide‐Capped Gold Nanoparticles: Towards Artificial Proteins , 2006, Chembiochem : a European journal of chemical biology.

[23]  Yoichiro Sato,et al.  Covalent immobilization of DNA on diamond and its verification by diffuse reflectance infrared spectroscopy , 2002 .

[24]  E. Nakamura,et al.  Functionalized fullerenes in water. The first 10 years of their chemistry, biology, and nanoscience. , 2003, Accounts of chemical research.

[25]  Chia-Liang Cheng,et al.  Biocompatible and detectable carboxylated nanodiamond on human cell , 2007 .

[26]  L. Liz‐Marzán,et al.  Plasmon coupling in layer-by-layer assembled gold nanorod films. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[27]  Huan-Cheng Chang,et al.  Bright fluorescent nanodiamonds: no photobleaching and low cytotoxicity. , 2005, Journal of the American Chemical Society.

[28]  Hsiao-Yun Wu,et al.  Characterization and application of single fluorescent nanodiamonds as cellular biomarkers , 2007, Proceedings of the National Academy of Sciences.

[29]  C. Cheng,et al.  Size-dependent surface CO stretching frequency investigations on nanodiamond particles. , 2006, The Journal of chemical physics.