Pigment Pattern Formation by Contact-Dependent Depolarization

Cell culture experiments reveal that direct interactions between pigment cells play a key role in skin pattern formation. Although recent experimental studies have suggested that the interactions among the pigment cells play a key role in the skin pattern formation, details of the mechanism remain largely unknown. By using an in vitro cell culture system, we have detected interactions between the two pigment cell types, melanophores and xanthophores, in the zebrafish skin. During primary culture, the melanophore membrane transiently depolarizes when contacted with the dendrites of a xanthophore. This depolarization triggers melanophore migration to avoid further contact with the xanthophores. Cell depolarization and repulsive movement were not observed in pigment cells with the jaguar mutant, which shows defective segregation of melanophores and xanthophores. The depolarization-repulsion of wild-type pigment cells may explain the pigment cell behaviors generating the stripe pattern of zebrafish.

[1]  Stephen L. Johnson,et al.  Pigment Pattern in jaguar/obelix Zebrafish Is Caused by a Kir7.1 Mutation: Implications for the Regulation of Melanosome Movement , 2006, PLoS genetics.

[2]  J. M. Turner,et al.  Temporal and cellular requirements for Fms signaling during zebrafish adult pigment pattern development , 2003, Development.

[3]  Shigeru Kondo,et al.  Melanophores in the stripes of adult zebrafish do not have the nature to gather, but disperse when they have the space to move , 2008, Pigment cell & melanoma research.

[4]  Shigeru Kondo,et al.  Interactions between zebrafish pigment cells responsible for the generation of Turing patterns , 2009, Proceedings of the National Academy of Sciences.

[5]  H. Meinhardt.  Models of biological pattern formation , 1982 .

[6]  C. Nüsslein-Volhard,et al.  Formation of the adult pigment pattern in zebrafish requires leopard and obelix dependent cell interactions , 2003, Development.

[7]  C. Nüsslein-Volhard,et al.  Zebrafish pigmentation mutations and the processes of neural crest development. , 1996, Development.

[8]  Akihiro Urasaki,et al.  Functional Dissection of the Tol2 Transposable Element Identified the Minimal cis-Sequence and a Highly Repetitive Sequence in the Subterminal Region Essential for Transposition , 2006, Genetics.

[9]  Kazuharu Furutani,et al.  Inwardly rectifying potassium channels: their structure, function, and physiological roles. , 2010, Physiological reviews.

[10]  D. Epps,et al.  Characterization of the steady-state and dynamic fluorescence properties of the potential-sensitive dye bis-(1,3-dibutylbarbituric acid)trimethine oxonol (Dibac4(3)) in model systems and cells. , 1994, Chemistry and physics of lipids.

[11]  Masaru Ishii,et al.  Spot pattern of leopard Danio is caused by mutation in the zebrafish connexin41.8 gene , 2006, EMBO reports.

[12]  D. Raible,et al.  Direct regulation of nacre, a zebrafish MITF homolog required for pigment cell formation, by the Wnt pathway. , 2000, Genes & development.

[13]  A. Turing.  The chemical basis of morphogenesis , 1952, Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences.