Myosin concentration underlies cell size–dependent scalability of actomyosin ring constriction

The rate of actomyosin ring constriction in cells of different sizes correlates with myosin motor concentration in Neurospora crassa cells, leading to increased division rates in larger cells during cytokinesis.

[1]  Rowland H. Davis,et al.  [4] Genetic and microbiological research techniques for Neurospora crassa , 1970 .

[2]  Alexander Lichius,et al.  F-Actin Dynamics in Neurospora crassa , 2010, Eukaryotic Cell.

[3]  T. Pollard,et al.  Arrangement of actin filaments and myosin-like filaments in the contractile ring and of actin-like filaments in the mitotic spindle of dividing HeLa cells. , 1986, Journal of ultrastructure and molecular structure research.

[4]  A. Stewart,et al.  Skeletal Muscle Performance Determined by Modulation of Number of Myosin Motors Rather Than Motor Force or Stroke Size , 2007, Cell.

[5]  Masayuki Yamamoto,et al.  Type II Myosin Heavy Chain Encoded by the myo2 Gene Composes the Contractile Ring during Cytokinesis in Schizosaccharomyces pombe , 1997, The Journal of cell biology.

[6]  F. Barr,et al.  Cytokinesis: Placing and Making the Final Cut , 2007, Cell.

[7]  Hua Wei,et al.  The important role of actinin-like protein (AcnA) in cytokinesis and apical dominance of hyphal cells in Aspergillus nidulans. , 2009, Microbiology.

[8]  Timothy J Mitchison,et al.  Dissecting Temporal and Spatial Control of Cytokinesis with a Myosin II Inhibitor , 2003, Science.

[9]  M. Balasubramanian,et al.  Type II myosin regulatory light chain relieves auto-inhibition of myosin-heavy-chain function , 2000, Nature Cell Biology.

[10]  R. Zucker,et al.  Quality assessment of confocal microscopy slide based systems: Performance , 2006, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[11]  S. Ono,et al.  Tropomyosin and troponin are required for ovarian contraction in the Caenorhabditis elegans reproductive system. , 2004, Molecular biology of the cell.

[12]  I. Mabuchi,et al.  The effect of myosin antibody on the division of starfish blastomeres , 1977, The Journal of cell biology.

[13]  Sergio A. Rincon,et al.  Schizosaccharomyces pombe Pxl1 is a paxillin homologue that modulates Rho1 activity and participates in cytokinesis. , 2008, Molecular biology of the cell.

[14]  Masako Osumi,et al.  Three-dimensional arrangement of F-actin in the contractile ring of fission yeast , 2007, The Journal of cell biology.

[15]  Michael Unser,et al.  A pyramid approach to subpixel registration based on intensity , 1998, IEEE Trans. Image Process..

[16]  D. Helfman,et al.  A new tropomyosin essential for cytokinesis in the fission yeast S. pombe , 1992, Nature.

[17]  Michael Freitag,et al.  GFP as a tool to analyze the organization, dynamics and function of nuclei and microtubules in Neurospora crassa. , 2004, Fungal genetics and biology : FG & B.

[18]  Marileen Dogterom,et al.  Direct measurement of force generation by actin filament polymerization using an optical trap , 2007, Proceedings of the National Academy of Sciences.

[19]  J. Spudich,et al.  Gene replacement in Dictyostelium: generation of myosin null mutants. , 1989, The EMBO journal.

[20]  G. Oster,et al.  Shrinking Gels Pull Cells , 2003, Science.

[21]  A. Kimura,et al.  Cell-Size-Dependent Spindle Elongation in the Caenorhabditis elegans Early Embryo , 2009, Current Biology.

[22]  D. Robinson,et al.  Balance of actively generated contractile and resistive forces controls cytokinesis dynamics. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[23]  J. Hamer,et al.  SEPH, a Cdc7p orthologue from Aspergillus nidulans, functions upstream of actin ring formation during cytokinesis , 2001, Molecular microbiology.

[24]  K. Gould,et al.  Requirements of fission yeast septins for complex formation, localization, and function. , 2004, Molecular biology of the cell.

[25]  J. Hamer,et al.  The Aspergillus nidulans sepA gene encodes an FH1/2 protein involved in cytokinesis and the maintenance of cellular polarity , 1997, The EMBO journal.

[26]  E. Mauceli,et al.  The genome sequence of the filamentous fungus Neurospora crassa , 2003, Nature.

[27]  G. Oster,et al.  Cell biology. Shrinking gels pull cells. , 2003, Science.

[28]  T. Holak,et al.  Lifeact: a versatile marker to visualize F-actin , 2008, Nature Methods.

[29]  R. Waterston,et al.  Cloning, sequencing, and mapping of an α-actinin gene from the nematode Caenorhabditis elegans , 1991 .

[30]  T. Kachur,et al.  Maternal UNC-45 is involved in cytokinesis and colocalizes with non-muscle myosin in the early Caenorhabditis elegans embryo , 2004, Journal of Cell Science.

[31]  Thomas D Pollard,et al.  Counting Cytokinesis Proteins Globally and Locally in Fission Yeast , 2005, Science.

[32]  M. Balasubramanian,et al.  Rng2p, a protein required for cytokinesis in fission yeast, is a component of the actomyosin ring and the spindle pole body , 1998, Current Biology.

[33]  N. Jones,et al.  Type II myosin involved in cytokinesis in the fission yeast, Schizosaccharomyces pombe. , 1997, Cell motility and the cytoskeleton.

[34]  Fred Chang,et al.  Actin dynamics in the contractile ring during cytokinesis in fission yeast , 2002, Nature.

[35]  A. Reymond,et al.  The S. pombe cdc15 gene is a key element in the reorganization of F-actin at mitosis , 1995, Cell.

[36]  Guy Cavet,et al.  Quantitation of the distribution and flux of myosin-II during cytokinesis , 2002, BMC Cell Biology.

[37]  J. Demeter,et al.  imp2, a New Component of the Actin Ring in the Fission Yeast Schizosaccharomyces pombe , 1998, The Journal of cell biology.

[38]  J. Demeter,et al.  imp 2 , a New Component of the Actin Ring in the Fission Yeast Schizosaccharomyces pombe , 1998 .

[39]  D. Baillie,et al.  A Formin Homology Protein and a Profilin Are Required for Cytokinesis and Arp2/3-Independent Assembly of Cortical Microfilaments in C. elegans , 2002, Current Biology.

[40]  Gregory Jedd,et al.  A Tether for Woronin Body Inheritance Is Associated with Evolutionary Variation in Organelle Positioning , 2009, PLoS genetics.

[41]  Thomas D Pollard,et al.  Spatial and temporal pathway for assembly and constriction of the contractile ring in fission yeast cytokinesis. , 2003, Developmental cell.

[42]  W. Loomis,et al.  Developmental consequences of the lack of myosin heavy chain in Dictyostelium discoideum. , 1988, Developmental biology.

[43]  F. Piano,et al.  Diverse roles of actin in C. elegans early embryogenesis , 2007, BMC Developmental Biology.

[44]  J. Bähler,et al.  Roles of a fimbrin and an alpha-actinin-like protein in fission yeast cell polarization and cytokinesis. , 2001, Molecular biology of the cell.

[45]  P. Wadsworth,et al.  Myosin-II-Dependent Localization and Dynamics of F-Actin during Cytokinesis , 2005, Current Biology.

[46]  J. Spudich,et al.  Disruption of the Dictyostelium myosin heavy chain gene by homologous recombination. , 1987, Science.

[47]  R. Waterston,et al.  Cloning, sequencing, and mapping of an alpha-actinin gene from the nematode Caenorhabditis elegans. , 1991, Cell motility and the cytoskeleton.

[48]  N. L. Glass,et al.  A Rho-Type GTPase, rho-4, Is Required for Septation in Neurospora crassa , 2005, Eukaryotic Cell.

[49]  M. Balasubramanian,et al.  Pxl1p, a paxillin-related protein, stabilizes the actomyosin ring during cytokinesis in fission yeast. , 2008, Molecular biology of the cell.

[50]  Yu-Li Wang,et al.  Cortical Actin Turnover during Cytokinesis Requires Myosin II , 2005, Current Biology.

[51]  Paul Nurse,et al.  Genetic control of cell size at cell division in yeast , 1975, Nature.

[52]  V. Simanis,et al.  The S. pombe rlc1 gene encodes a putative myosin regulatory light chain that binds the type II myosins myo3p and myo2p. , 2000, Journal of cell science.

[53]  M. Lord,et al.  Tropomyosin and Myosin-II Cellular Levels Promote Actomyosin Ring Assembly in Fission Yeast , 2010, Molecular biology of the cell.

[54]  K. Gould,et al.  The Schizosaccharomyces pombe cdc3+ gene encodes a profilin essential for cytokinesis , 1994, The Journal of cell biology.

[55]  K. Nakano,et al.  Actin-depolymerizing protein Adf1 is required for formation and maintenance of the contractile ring during cytokinesis in fission yeast. , 2006, Molecular biology of the cell.

[56]  B. Bowerman,et al.  The Nonmuscle Myosin Regulatory Light Chain Gene mlc-4 Is Required for Cytokinesis, Anterior-Posterior Polarity, and Body Morphology during Caenorhabditis elegans Embryogenesis , 1999, The Journal of cell biology.

[57]  Marie-France Carlier,et al.  Mechanism of Actin-Based Motility , 2001, Science.

[58]  W. Sullivan,et al.  Characterization of anillin mutants reveals essential roles in septin localization and plasma membrane integrity , 2005, Development.

[59]  J. White,et al.  The C. elegans septin genes, unc-59 and unc-61, are required for normal postembryonic cytokineses and morphogenesis but have no essential function in embryogenesis. , 2000, Journal of cell science.

[60]  J. Spudich,et al.  Determinants of Myosin II Cortical Localization during Cytokinesis , 2010, Current Biology.

[61]  R. Lew.  Mass flow and pressure-driven hyphal extension in Neurospora crassa. , 2005, Microbiology.

[62]  J. Ishiguro,et al.  An actin point‐mutation neighboring the ‘hydrophobic plug’ causes defects in the maintenance of cell polaroty and septum organization in the fission yeast Schizosaccharomyces pombe , 1996, FEBS letters.

[63]  J. Yates,et al.  Dissection of the Mammalian Midbody Proteome Reveals Conserved Cytokinesis Mechanisms , 2004, Science.

[64]  M. Marra,et al.  The Caenorhabditis elegans unc-60 gene encodes proteins homologous to a family of actin-binding proteins , 1994, Molecular and General Genetics MGG.

[65]  Karen Oegema,et al.  Structural Memory in the Contractile Ring Makes the Duration of Cytokinesis Independent of Cell Size , 2009, Cell.

[66]  E. Bi,et al.  Comparative Analysis of Cytokinesis in Budding Yeast, Fission Yeast and Animal Cells , 2004, Current Biology.

[67]  K. Gould,et al.  Isolation and characterization of new fission yeast cytokinesis mutants. , 1998, Genetics.

[68]  S. Yumura.  Myosin II dynamics and cortical flow during contractile ring formation in Dictyostelium cells , 2001, The Journal of cell biology.

[69]  K. Gould,et al.  Schizosaccharomyces pombe cdc4+ gene encodes a novel EF-hand protein essential for cytokinesis , 1995, The Journal of cell biology.

[70]  Anthony A. Hyman,et al.  Supporting Material : Stress Generation and Filament Turnover During Actin Ring Constriction , 2007 .

[71]  Fred Chang,et al.  cdc12p, a Protein Required for Cytokinesis in Fission Yeast, Is a Component of the Cell Division Ring and Interacts with Profilin , 1997, The Journal of cell biology.

[72]  Patrick C. Hickey,et al.  Live-cell Imaging of filamentous fungi using vital fluorescent dyes and confocal microscopy , 2004 .

[73]  A. Kimball,et al.  CONTROL OF NUCLEAR RATIOS IN NEUROSPORA HETEROKARYONS , 1955 .

[74]  J. Sellers,et al.  Mechanism of Blebbistatin Inhibition of Myosin II* , 2004, Journal of Biological Chemistry.

[75]  V. Simanis,et al.  The S . pombe rlc 1 gene encodes a putative myosin regulatory light chain that binds the type II myosins myo 3 p and myo 2 p , 2000 .