Changes in markers of oxidative stress and membrane properties in synaptosomes from rats exposed prenatally to toluene.

The present study was undertaken in order to investigate if toluene induced oxidative stress in brains from rats exposed prenatally to 1800 ppm toluene 6 hr/day at days 7-20 during the pregnancy. 35-42 days after birth the rats were killed and synaptosomal fractions were prepared for the experiments. Synaptosomes from rats exposed prenatally to toluene exhibited an increased level of oxidative stress when incubated with toluene in vitro compared to synaptosomes from unexposed offspring. Also the cell membrane was affected, as the calcium leakage was more increased from exposed synaptosomes than from unexposed. The membrane fluidity increased significantly when synaptosomes were incubated with toluene for 10 min. in vitro but the change in fluidity was identical in both groups of offspring. The results indicate that prenatal exposure to toluene induces long-lasting changes in oxidative status and membrane function.

[1]  R. Sarangarajan,et al.  Functional impairment of renal organic cation transport in experimental diabetes. , 2002, Pharmacology & toxicology.

[2]  C. Chinopoulos,et al.  Depolarization of In Situ Mitochondria Due to Hydrogen Peroxide‐Induced Oxidative Stress in Nerve Terminals , 1999, Journal of neurochemistry.

[3]  U. Hass,et al.  Developmental neurotoxicity after toluene inhalation exposure in rats. , 1999, Neurotoxicology and teratology.

[4]  J. Lemasters,et al.  Contribution of increased mitochondrial free Ca2+ to the mitochondrial permeability transition induced by tert‐butylhydroperoxide in rat hepatocytes , 1999, Hepatology.

[5]  U. Hass,et al.  The effect of in vitro exposure to white spirit on [Ca2+i] in synaptosomes from rats exposed prenatally to white spirit. , 1999, Pharmacology & toxicology.

[6]  U. Hass,et al.  Effects of prenatal exposure to xylene on postnatal development and behavior in rats. , 1999, Neurotoxicology and teratology.

[7]  G. Cortopassi,et al.  Mitochondria in organismal aging and degeneration. , 1999, Biochimica et biophysica acta.

[8]  A. Vercesi,et al.  Mitochondrial damage induced by conditions of oxidative stress. , 1999, Free radical biology & medicine.

[9]  M. Mattson,et al.  Pivotal Role of Mitochondrial Calcium Uptake in Neural Cell Apoptosis and Necrosis , 1999, Journal of neurochemistry.

[10]  B. Ames,et al.  Mitochondrial Decay in Aging: Reversal through Supplementation of Acetyl‐l‐Carnitine and N‐tert‐Butyl‐α‐phenyl‐nitrone a , 1998, Annals of the New York Academy of Sciences.

[11]  B. Ames,et al.  Mitochondrial Aging: Open Questions a , 1998, Annals of the New York Academy of Sciences.

[12]  C. Colton,et al.  Ethanol induced changes in superoxide anion and nitric oxide in cultured microglia. , 1998, Alcoholism, clinical and experimental research.

[13]  S. Gospe,et al.  Toluene abuse embryopathy: longitudinal neurodevelopmental effects of prenatal exposure to toluene in rats. , 1998, Reproductive toxicology.

[14]  H. Jones,et al.  Inhalant abuse in pregnancy. , 1998, Obstetrics and gynecology clinics of North America.

[15]  P. Kaplan,et al.  Iron-Induced Inhibition of Na+, K+-ATPase and Na+/Ca2+ Exchanger in Synaptosomes: Protection by the Pyridoindole Stobadine , 1997, Neurochemical Research.

[16]  J. Adams,et al.  The effects of aging and neurodegeneration on apoptosis-associated DNA fragmentation and the benefits of nicotinamide. , 1997, Molecular and chemical neuropathology.

[17]  H. Jones,et al.  Neurobehavioral consequences of intermittent prenatal exposure to high concentrations of toluene. , 1997, Neurotoxicology and teratology.

[18]  M. Matsuo,et al.  Oxidative injury of synapse and alteration of antioxidative defense systems in rats, and its prevention by vitamin E. , 1997, European journal of biochemistry.

[19]  T. Tabatabaie,et al.  Inactivation of glutathione peroxidase by benzaldehyde. , 1996, Toxicology and applied pharmacology.

[20]  K. Sulik,et al.  Free radicals and ethanol-induced cytotoxicity in neural crest cells. , 1996, Alcoholism, clinical and experimental research.

[21]  S. Gospe,et al.  Development of a Rat Model of Toluene-Abuse Embryopathy , 1996, Pediatric Research.

[22]  U. Hass,et al.  The effect of ageing and in vitro exposure to xylene and KCl on [Ca2+]i in synaptosomes from rats exposed prenatally to xylene. , 1996, Pharmacology & toxicology.

[23]  M. Lindbohm.  Effects of Parental Exposure to Solvents on Pregnancy Outcome , 1995, Journal of occupational and environmental medicine.

[24]  U. Hass,et al.  Effects of prenatal exposure to toluene on postnatal development and behavior in rats. , 1995, Neurotoxicology and teratology.

[25]  H. Hänninen,et al.  Symptoms indicative of the effects of organic solvent exposure in Dutch painters. , 1994, Neurotoxicology and teratology.

[26]  H. Taskinen,et al.  Laboratory work and pregnancy outcome. , 1994, Journal of occupational medicine. : official publication of the Industrial Medical Association.

[27]  O. Ladefoged,et al.  Three weeks' exposure of rats to dearomatized white spirit modifies indices of oxidative stress in brain, kidney, and liver. , 1994, Biochemical pharmacology.

[28]  Pears Ma,et al.  Toluene Embryopathy: Delineation of the Phenotype and Comparison With Fetal Alcohol Syndrome , 1994 .

[29]  U. Hass,et al.  Prenatal toxicity of xylene inhalation in the rat: a teratogenicity and postnatal study. , 1993, Pharmacology & toxicology.

[30]  S. Ali,et al.  Toluene-induced oxidative stress in several brain regions and other organs. , 1993, Molecular and chemical neuropathology.

[31]  S. Edelfors,et al.  Effect of organic solvents on nervous cell membrane as measured by changes in the (Ca2+/Mg2+) ATPase activity and fluidity of synaptosomal membrane. , 1992, Pharmacology & toxicology.

[32]  K. Reuhl.  Delayed expression of neurotoxicity: the problem of silent damage. , 1991, Neurotoxicology.

[33]  S. Bondy,et al.  Sensitive and rapid quantitation of oxygen reactive species formation in rat synaptosomes , 1990, Neurochemistry International.

[34]  M. Mckee,et al.  The effect of oxidative stress on levels of cytosolic calcium within and uptake of calcium by synaptosomes , 1990, Neurochemistry International.

[35]  T. R. Riul,et al.  Developmental toxicity of in utero exposure to toluene on malnourished and well nourished rats. , 1990, Toxicology.

[36]  J. Rungby,et al.  The effect of low-level toluene exposure on the developing hippocampal region of the rat: histological evidence and volumetric findings. , 1990, Toxicology.

[37]  G Triebig,et al.  Occupational neurotoxicology of organic solvents and solvent mixtures. , 1989, Neurotoxicology and teratology.

[38]  J. Hersh.  Toluene embryopathy: two new cases. , 1989, Journal of medical genetics.

[39]  L. de Meis,et al.  Activation of the purified erythrocyte plasma membrane Ca2+‐ATPase by organic solvents , 1989, FEBS letters.

[40]  M. McHugh.  The abuse of volatile substances. , 1987, Pediatric clinics of North America.

[41]  G. Porcellati.  DISTRIBUTION AND METABOLISM OF THREONINE ETHANOLAMINE AND SERINE ETHANOLAMINE PHOSPHODIESTERS IN NERVOUS TISSUES. , 1964, Biochimica et biophysica acta.

[42]  Oliver H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[43]  G. Ellison,et al.  Prenatal phencyclidine induces heightened neurodegeneration in rats in some brain regions, especially during 2nd trimester, but possible anti-apoptotic effects in others. , 2002, Pharmacology & toxicology.

[44]  U. Hass,et al.  Long-lasting neurobehavioral effects of prenatal exposure to xylene in rats. , 1997, Neurotoxicology.

[45]  U. Hass,et al.  Dearomatized white spirit inhalation exposure causes long-lasting neurophysiological changes in rats. , 1996, Neurotoxicology and teratology.

[46]  O. Ladefoged,et al.  Report on a conference on organic solvents and the nervous system. , 1992, Neurotoxicology and teratology.

[47]  P. Brown-Woodman,et al.  Embryotoxicity of xylene and toluene: an in vitro study. , 1991, Industrial health.

[48]  H. Tähti,et al.  The mechanism of the anaesthetic action of organic solvents studied with different membrane models in vitro. , 1991, Archives of toxicology. Supplement. = Archiv fur Toxikologie. Supplement.

[49]  H. Gjerde,et al.  Driving under the influence of toluene. , 1990, Forensic science international.

[50]  M. Korpela.  Organic Solvents Affect Rat Synaptosome Membrane Acetylcholinesterase and Adenosine Triphosphatase In Vitro , 1988 .

[51]  B. Danielsson,et al.  Placental transfer and distribution of toluene, xylene and benzene, and their metabolites during gestation in mice. , 1986, Biological research in pregnancy and perinatology.

[52]  E. Tátrai,et al.  On the embryotoxic effects of benzene and its alkyl derivatives in mice, rats and rabbits. , 1985, Archives of toxicology. Supplement. = Archiv fur Toxikologie. Supplement.

[53]  G. Ungváry,et al.  Embryotoxic effects of benzene and its methyl derivatives: toluene, xylene. , 1978, Toxicology.

[54]  V. P. Whittaker,et al.  The isolation of nerve endings from brain: an electron-microscopic study of cell fragments derived by homogenization and centrifugation. , 1962, Journal of anatomy.