Predominant cultivable microflora of plaque on removable dentures in patients with denture-induced stomatitis.

Plaque from the fitting surface of upper full dentures in 8 patients with denture-induced stomatitis was sampled with curettes for study. To characterize the predominant cultivable flora, 1249 isolates (138–196 from each sample) were sub-cultured from anaerobic roll tubes. Streptococci constituted 17–76% of the isolates in each sample (median 29%) and were identified as S. mitior, S. milleri, S. mutans, S. salivarius, and S. sanguis. Staphylococci (mainly S. aureus) made up 2–15%, median 6%. Gram-positive rods constituted 5–72% (median 45%), mainly Lactobacillus spp. (0–72%, median 19%) and Actinomyces spp. (0–43%, median 9%). Gram-negative cocci made up 0–28% (median 15%) and Gram-negative rods only 0–1.8% (median 0%). Yeast viable counts on Sabouraud agar corresponded to 0–1.7% (median 0.27%) of the total viable counts. Stomatitis-inducing denture plaque has a similar complex and variable, mainly Gram-positive, bacterial flora as that of denture plaque in subjects with healthy oral mucosa. Yeasts and lactobacilli are, however, in greater evidence in stomatitis.

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