Pretreatment of wheat straw using steam, steam/acetic acid and steam/ethanol and its enzymatic hydrolysis for sugar production

Wheat straw is a suitable raw material for ethanol production, since it has a high cellulose content. In the bioconversion of lignocellulosic materials to ethanol, pretreatment of the material prior to enzymatic hydrolysis is essential to obtain high overall yields of sugar and ethanol. The objective here is to find additives which increase the efficiency of the sugar production. The pretreatment of wheat straw by steam explosion soaked with acetic acid or ethanol prior to the pretreatment was investigated by varying the temperature (180–225) °C and the retention time (3–60 min). The results showed that the pretreatment of wheat straw by steam explosion with soaked with acetic acid or ethanol was more effective than that by steam explosion alone. Additionally, the pretreatment of wheat straw by steam explosion at 210 °C and 10 min, by steam/acetic acid at 220 °C and 8 min and by steam/ethanol at 220 °C and 5 min resulted in the best overall yield of reducing sugar which was found to be 177.3 g kg−1 on dry solids (DS) basis, 244.1 g kg−1 DS, and 264.3 g kg−1 DS, respectively.

[1]  J N Saddler,et al.  Steam‐explosion pretreatment of wood: Effect of chip size, acid, moisture content and pressure drop , 1986, Biotechnology and bioengineering.

[2]  J. Gressel,et al.  Effects of aromatic compounds on hemicellulose‐degrading enzymes in Aspergillus japonicus , 1985, Biotechnology and Bioengineering.

[3]  Ye Sun,et al.  Hydrolysis of lignocellulosic materials for ethanol production: a review. , 2002, Bioresource technology.

[4]  M. Rebeller,et al.  Inhibition of Rumen microbes by compounds formed in the steam treatment of wheat straw , 1994 .

[5]  L. Spano,et al.  Technology advances for continuous compression milling pretreatment of lignocellulosics for enzymatic hydrolysis , 1982, Biotechnology and bioengineering.

[6]  M. Playne,et al.  Increased digestibility of bagasses by pretreatment with alkalis and steam explosion , 1984, Biotechnology and bioengineering.

[7]  Chen Hongzhang,et al.  Unpolluted fractionation of wheat straw by steam explosion and ethanol extraction. , 2007, Bioresource technology.

[8]  M. Vignon,et al.  Steam explosion of woody hemp chènevotte. , 1995, International journal of biological macromolecules.

[9]  G. Zeeman,et al.  Pretreatments to enhance the digestibility of lignocellulosic biomass. , 2009, Bioresource technology.

[10]  B. Saha,et al.  Dilute acid pretreatment, enzymatic saccharification and fermentation of wheat straw to ethanol , 2005 .

[11]  Guido Zacchi,et al.  Simultaneous detoxification and enzyme production of hemicellulose hydrolysates obtained after steam pretreatment , 1997 .

[12]  F. Castro THE USE OF STEAM TREATMENT TO UPGRADE LIGNOCELLULOSIC MATERIALS FOR ANIMAL FEED , 1994 .

[13]  Eulogio Castro,et al.  Evaluation of steam explosion pre-treatment for enzymatic hydrolysis of sunflower stalks. , 2008, Enzyme and microbial technology.

[14]  G. Zacchi,et al.  Enzymatic hydrolysis of steam-pretreated lignocellulosic materials with Trichoderma atroviride enzymes produced in-house , 2009, Biotechnology for biofuels.

[15]  M. Ballesteros,et al.  Effect of lignocellulosic degradation compounds from steam explosion pretreatment on ethanol fermentation by thermotolerant yeast Kluyveromyces marxianus , 2003 .

[16]  Bärbel Hahn-Hägerdal,et al.  Fermentation of lignocellulosic hydrolysates. II: inhibitors and mechanisms of inhibition. , 2000 .

[17]  V. C. Badve,et al.  Effect of high-pressure steam treatment on chemical composition and digestibility in vitro of roughages , 1982 .

[18]  T. K. Ghose Measurement of cellulase activities , 1987 .

[19]  Liisa Viikari,et al.  Evaluation of wet oxidation pretreatment for enzymatic hydrolysis of softwood , 2004, Applied biochemistry and biotechnology.

[20]  Shabbir H. Gheewala,et al.  Energy balance and GHG-abatement cost of cassava utilization for fuel ethanol in Thailand , 2007 .

[21]  Mohammad J. Taherzadeh,et al.  Ethanol production from dilute-acid pretreated rice straw by simultaneous saccharification and fermentation with Mucor indicus, Rhizopus oryzae, and Saccharomyces cerevisiae , 2006 .

[22]  J. Fernández-Bolaños,et al.  Steam-explosion of olive stones: hemicellulose solubilization and enhancement of enzymatic hydrolysis of cellulose. , 2001, Bioresource technology.

[23]  B. Saha,et al.  Ethanol Production from Alkaline Peroxide Pretreated Enzymatically Saccharified Wheat Straw , 2006, Biotechnology progress.

[24]  H. Schellhorn,et al.  The release of fermentable carbohydrate from peat by steam explosion and its use in the microbial production of solvents , 1986, Biotechnology and bioengineering.

[25]  R. Sun,et al.  Characteristics of degraded cellulose obtained from steam-exploded wheat straw. , 2005, Carbohydrate research.

[26]  A. Voragen,et al.  Effect of pretreatment severity on xylan solubility and enzymatic breakdown of the remaining cellulose from wheat straw. , 2007, Bioresource technology.

[27]  E. Chornet,et al.  Application of steam explosion to the fractionation and rapid vapor-phase alkaline pulping of wheat straw , 1998 .

[28]  Anne Belinda Thomsen,et al.  Study of the phenolic compounds formed during pretreatment of sugarcane bagasse by wet oxidation and steam explosion , 2007 .

[29]  R. Britton Removal of the growth inhibitor(s) from acid and pressure hydrolyzed sawdust. , 1978, Journal of agricultural and food chemistry.

[30]  John A. Heitmann,et al.  Feedstock pretreatment strategies for producing ethanol from wood, bark, and forest residues , 2008, BioResources.

[31]  Maria Cantarella,et al.  Comparison of different detoxification methods for steam-exploded poplar wood as a substrate for the bioproduction of ethanol in SHF and SSF. , 2004 .

[32]  D. Gregg,et al.  A techno-economic assessment of the pretreatment and fractionation steps of a biomass-to-ethanol process , 1996 .

[33]  Foster A. Agblevor,et al.  Characterization and fermentation of steam exploded cotton gin waste , 2001 .

[34]  F. Kobayashi,et al.  Saccharification and alcohol fermentation in starch solution of steam-exploded potato , 1998, Applied biochemistry and biotechnology.

[35]  G. Zacchi,et al.  Optimization of steam pretreatment of corn stover to enhance enzymatic digestibility , 2004, Applied biochemistry and biotechnology.

[36]  H. Grethlein,et al.  The Effect of Pore Size Distribution on the Rate of Enzymatic Hydrolysis of Cellulosic Substrates , 1985, Bio/Technology.

[37]  Mats Galbe,et al.  The effect of water-soluble inhibitors from steam-pretreated willow on enzymatic hydrolysis and ethanol fermentation , 1996 .

[38]  L. Gustafsson,et al.  Characterization and fermentation of dilute-acid hydrolyzates from wood , 1997 .

[39]  E. Mounir,et al.  Characterization of Two Non Constitutive Hydroxycinnamic Acid Derivatives in Date Palm (Phoenix dactylifera L.) Callus in Relation with Tissue Browning , 2004 .

[40]  Lijun Wang,et al.  Effect of steam explosion on biodegradation of lignin in wheat straw. , 2008, Bioresource technology.

[41]  R. Sun,et al.  Influence of alkaline pre-treatments on the cell wall components of wheat straw , 1995 .

[42]  J. Saddler,et al.  The nature of inhibitory materials present in pretreated lignocellulosic substrates which inhibit the enzymatic hydrolysis of cellulose , 1983, Biotechnology Letters.

[43]  Hui Li,et al.  The effect of microwave irradiation on enzymatic hydrolysis of rice straw. , 2006, Bioresource technology.

[44]  G. L. Miller Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar , 1959 .

[45]  Honghua Jia,et al.  Dilute sulfuric acid cycle spray flow-through pretreatment of corn stover for enhancement of sugar recovery. , 2009, Bioresource technology.

[46]  Hongzhang Chen,et al.  Superfine grinding of steam-exploded rice straw and its enzymatic hydrolysis , 2006 .

[47]  Frank Taylor,et al.  Corn-milling pretreatment with anhydrous ammonia , 2003, Applied biochemistry and biotechnology.

[48]  Michael M. Wu,et al.  Optimization of steam explosion to enhance hemicellulose recovery and enzymatic hydrolysis of cellulose in softwoods , 1999 .

[49]  M. Taherzadeh,et al.  Pretreatment of Lignocellulosic Wastes to Improve Ethanol and Biogas Production: A Review , 2008, International journal of molecular sciences.

[50]  C. Hongzhang,et al.  Absorption of ethanol by steam-exploded corn stalk. , 2009, Bioresource technology.

[51]  Mats Galbe,et al.  Comparison of SO2 and H2SO4 impregnation of softwood prior to steam pretreatment on ethanol production , 1998 .