Use of in vivo phycocyanin fluorescence to monitor potential microcystin-producing cyanobacterial biovolume in a drinking water source.

The source water of a drinking water treatment plant prone to blooms, dominated by potential microcystin-producing cyanobacteria, was monitored for two seasons in 2007-2008. In the 2008 season, the median value for potential microcystin-producing cyanobacterial biovolume was 87% of the total phytoplankton biovolume in the untreated water of the plant. Depth profiles taken above the plant's intake identified three sampling days at high risk for the contamination of the plant's raw water with potentially toxic cyanobacteria. Chlorophyceae and Bacillariophyceae caused false positive values to be generated by the phycocyanin probe when cyanobacteria represented a small fraction of the total phytoplanktonic biovolume present. However, there was little interference with the phycocyanin probe readings by other algal species when potential microcystin-producing cyanobacteria dominated the phytoplankton of the plant's untreated water. A two-tiered method for source water monitoring, using in vivo phycocyanin fluorescence, is proposed based on (1) a significant relationship between in vivo phycocyanin fluorescence and cyanobacterial biovolume and (2) the calculated maximum potential microcystin concentration produced by dominant Microcystis sp. biovolume. This method monitors locally-generated threshold values for cyanobacterial biovolume and microcystin concentrations using in vivo phycocyanin fluorescence.

[1]  E. Testai,et al.  Human Health Risk Assessment Related to Cyanotoxins Exposure , 2008, Critical reviews in toxicology.

[2]  Hee-Mock Oh,et al.  Alternative alert system for cyanobacterial bloom, using phycocyanin as a level determinant. , 2007, Journal of microbiology.

[3]  F. Frimmel,et al.  Use of fluorescence fingerprints for the estimation of bloom formation and toxin production of Microcystis aeruginosa. , 2010, Water research.

[4]  J. Bartram,et al.  Health risks caused by freshwater cyanobacteria in recreational waters. , 2000, Journal of toxicology and environmental health. Part B, Critical reviews.

[5]  I. Baudin,et al.  A phycocyanin probe as a tool for monitoring cyanobacteria in freshwater bodies. , 2008, Journal of environmental monitoring : JEM.

[6]  C. Lorenzen,et al.  A method for the continuous measurement of in vivo chlorophyll concentration , 1966 .

[7]  M. Burch,et al.  The impact of conventional water treatment processes on cells of the cyanobacterium Microcystis aeruginosa , 1999 .

[8]  P. Orr,et al.  Release and degradation of microcystin following algicide treatment of a Microcystis aeruginosa bloom in a recreational lake, as determined by HPLC and protein phosphatase inhibition assay , 1994 .

[9]  B. Whitton,et al.  The Freshwater Algal Flora of the British Isles , 2021 .

[10]  Jukka Seppälä,et al.  Ship-of-opportunity based phycocyanin fluorescence monitoring of the filamentous cyanobacteria bloom dynamics in the Baltic Sea , 2007 .

[11]  A. Kathuria,et al.  Change in cyanobacterial biovolume due to preservation by Lugol's Iodine , 2005 .

[12]  K. Izydorczyk,et al.  Establishment of an Alert Level Framework for cyanobacteria in drinking water resources by using the Algae Online Analyser for monitoring cyanobacterial chlorophyll a. , 2009, Water research.

[13]  J. Lund,et al.  The inverted microscope method of estimating algal numbers and the statistical basis of estimations by counting , 1958, Hydrobiologia.

[14]  Klaus Koop,et al.  Eutrophication in Australian Rivers, Reservoirs and Estuaries – A Southern Hemisphere Perspective on the Science and its Implications , 2006, Hydrobiologia.

[15]  Y. Prairie,et al.  Empirical study of cyanobacterial toxicity along a trophic gradient of lakes , 2005 .

[16]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[17]  Shun-zhang Yu.  Primary prevention of hepatocellular carcinoma , 1995, Journal of gastroenterology and hepatology.

[18]  Blahoslav Marsálek,et al.  Detection and estimation of potentially toxic cyanobacteria in raw water at the drinking water treatment plant by in vivo fluorescence method. , 2007, Water research.

[19]  W. Carmichael,et al.  Human Fatalities from Cyanobacteria: Chemical and Biological Evidence for Cyanotoxins , 2001 .

[20]  Jürgen Weckesser,et al.  Microcystins (hepatotoxic heptapeptides) in german fresh water bodies , 1999 .

[21]  M. Watzin,et al.  Application of the WHO alert level framework to cyanobacterial monitoring of Lake Champlain, Vermont , 2006, Environmental toxicology.

[22]  K. Sivonen,et al.  Occurrence of microcystins in raw water sources and treated drinking water of Finnish waterworks. , 2001, Water science and technology : a journal of the International Association on Water Pollution Research.

[23]  H. Dau,et al.  A fluorometric method for the differentiation of algal populations in vivo and in situ , 2004, Photosynthesis Research.

[24]  S. Jacquet,et al.  Application of a submersible spectrofluorometer for rapid monitoring of freshwater cyanobacterial blooms: a case study , 2002 .

[25]  C. Reynolds.  The Ecology of Phytoplankton , 2006 .