Interspersed repetitive sequence (IRS)-PCR for typing of whole genome radiation hybrid panels.

The typing of a radiation hybrid (RH) panel is generally achieved using a unique primer pair for each marker. We here describe a complementing approach utilizing IRS-PCR. Advantages of this technology include the use of a single universal primer to specify any locus, the rapid typing of RH lines by hybridization, and the conservative use of hybrid DNA. The technology allows the mapping of a clone without the requirement for STS generation. To test the technique, we have mapped 48 BAC clones derived from mouse chromosome 12 which we mostly identified using complex probes. As mammalian genomes are repeat-rich, the technology can easily be adapted to species other than mouse.

[1]  H. Lehrach,et al.  Interspersed repetitive element polymerase chain reaction product mapping using a mouse interspecific backcross. , 1991, Genomics.

[2]  Denis Milan,et al.  A first-generation porcine whole-genome radiation hybrid map , 1999, Mammalian Genome.

[3]  D. Milan,et al.  Construction of a whole-genome radiation hybrid panel for high-resolution gene mapping in pigs , 1998, Cytogenetic and Genome Research.

[4]  F. Galibert,et al.  A whole-genome radiation hybrid map of the dog genome. , 1998, Genomics.

[5]  Donna K. Slonim,et al.  Radiation hybrid map of the mouse genome , 1999, Nature Genetics.

[6]  R. Myers,et al.  Radiation hybrid mapping: a somatic cell genetic method for constructing high-resolution maps of mammalian chromosomes. , 1990, Science.

[7]  H. Himmelbauer,et al.  Technology development at the interface of proteome research and genomics: Mapping nonpolymorphic proteins on the physical map of mouse chromosomes , 1999, Electrophoresis.

[8]  F. Galibert,et al.  Construction and optimization of a dog whole-genome radiation hybrid panel , 1999, Mammalian Genome.

[9]  Steve D. M. Brown,et al.  A YAC-based physical map of the mouse genome , 1999, Nature Genetics.

[10]  S. Goss,et al.  New method for mapping genes in human chromosomes , 1975, Nature.

[11]  R. Elliott,et al.  A radiation hybrid map of mouse chromosome 13. , 1999, Genomics.

[12]  J. W. Foster,et al.  Construction of a mouse whole-genome radiation hybrid panel and application to MMU11. , 1996, Genomics.