Heterochronic genes control the stage-specific initiation and expression of the dauer larva developmental program in Caenorhabditis elegans.

We report that a stage-specific developmental program, dauer larva formation, is temporally regulated by four heterochronic genes, lin-4, lin-14, lin-28, and lin-29. The effects of mutations in these four genes on dauer larva formation have revealed that they regulate two different processes of dauer larva formation: (1) a decision specifying the larval stage at which dauer larva development initiates, and (2) the specialized differentiation of hypodermal cells during dauer larva morphogenesis. Epistasis analysis has suggested a model in which lin-4 negatively regulates lin-14, and the resulting temporal decrease in lin-14 activity specifies the stage of dauer larva initiation. Our results further suggest that dauer larva morphogenesis by hypodermal cells requires that lin-28 acts to inhibit lin-29 during early larval stages.

[1]  G. Struhl,et al.  Differing strategies for organizing anterior and posterior body pattern in Drosophila embryos , 1989, Nature.

[2]  Diethard Tautz,et al.  Posterior segmentation of the Drosophila embryo in the absence of a maternal posterior organizer gene , 1989, Nature.

[3]  Ruth Lehmann,et al.  The Drosophila posterior-group gene nanos functions by repressing hunchback activity , 1989, Nature.

[4]  V. Ambros.  A hierarchy of regulatory genes controls a larva-to-adult developmental switch in C. elegans , 1989, Cell.

[5]  Gary Ruvkun,et al.  The Caenorhabditis elegans heterochronic gene lin-14 encodes a nuclear protein that forms a temporal developmental switch , 1989, Nature.

[6]  B. Meyer,et al.  xol-1: A gene that controls the male modes of both sex determination and X chromosome dosage compensation in C. elegans , 1988, Cell.

[7]  P. Ingham.  The molecular genetics of embryonic pattern formation in Drosophila , 1988, Nature.

[8]  D. Riddle,et al.  Mutants of Caenorhabditis elegans that form dauer-like larvae. , 1988, Developmental biology.

[9]  H. Horvitz,et al.  The lin-14 locus of Caenorhabditis elegans controls the time of expression of specific postembryonic developmental events. , 1987, Genes & development.

[10]  J. N. Thomson,et al.  Mutant sensory cilia in the nematode Caenorhabditis elegans. , 1986, Developmental biology.

[11]  C. Kenyon.  A gene involved in the development of the posterior body region of C. elegans , 1986, Cell.

[12]  D L Riddle,et al.  The Caenorhabditis elegans dauer larva: developmental effects of pheromone, food, and temperature. , 1984, Developmental biology.

[13]  H. Horvitz,et al.  The lin-12 locus specifies cell fates in caenorhabditis elegans , 1983, Cell.

[14]  H. Horvitz,et al.  Gonadal cell lineages of the nematode Panagrellus redivivus and implications for evolution by the modification of cell lineage. , 1981, Developmental biology.

[15]  Susan J. Brown,et al.  Sensory control of dauer larva formation in Caenorhabditis elegans , 1981, The Journal of comparative neurology.

[16]  D. Riddle,et al.  Critical periods in the development of the Caenorhabditis elegans dauer larva. , 1981, Developmental biology.

[17]  D. Riddle,et al.  Interacting genes in nematode dauer larva formation , 1981, Nature.

[18]  Martin Chalfie,et al.  Mutations that lead to reiterations in the cell lineages of C. elegans , 1981, Cell.

[19]  B. S. Baker,et al.  Sex and the single cell. I. On the action of major loci affecting sex determination in Drosophila melanogaster. , 1980, Genetics.

[20]  D. Hirsh,et al.  The postembryonic cell lineages of the hermaphrodite and male gonads in Caenorhabditis elegans. , 1979, Developmental biology.

[21]  S. W. Emmons,et al.  Analysis of the constancy of DNA sequences during development and evolution of the nematode Caenorhabditis elegans. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[22]  S. Brenner,et al.  Nondisjunction Mutants of the Nematode CAENORHABDITIS ELEGANS. , 1979, Genetics.

[23]  E. Lewis.  A gene complex controlling segmentation in Drosophila , 1978, Nature.

[24]  S. Gould,et al.  Ontogeny and Phylogeny , 1978 .

[25]  J. Hodgkin,et al.  Specific neuroanatomical changes in chemosensory mutants of the nematode Caenorhabditis elegans , 1977, The Journal of comparative neurology.

[26]  J. Sulston,et al.  Post-embryonic cell lineages of the nematode, Caenorhabditis elegans. , 1977, Developmental biology.

[27]  R. Cassada,et al.  The dauerlarva, a post-embryonic developmental variant of the nematode Caenorhabditis elegans. , 1975, Developmental biology.

[28]  S. Brenner.  The genetics of Caenorhabditis elegans. , 1974, Genetics.

[29]  J. Hodgkin.  Males, hermaphrodites and females: sex determination in Caenorhabbits elegans , 1985 .

[30]  P. Deák,et al.  Isolation and phenocritical period-analysis of conditional and non-conditional developmental mutants in Caenorhabditis elegans , 1981 .

[31]  S. Brenner,et al.  NONDISJUNCTION MUTANTS OF THE NEMATODE , 1979 .

[32]  R. K. Herman.  Crossover suppressors and balanced recessive lethals in Caenorhabditis elegans. , 1978, Genetics.

[33]  A. L. Taylor,et al.  Biology, identification and control of root-knot nematodes (Meloidogyne species). , 1978 .

[34]  J. Sulston,et al.  Some Observations On Moulting in Caenorhabditis Elegans , 1978 .

[35]  D. Riddle.  A genetic pathway for dauer larva formation in caenorhabditis elegans : (nematode, development, neuron) , 1977 .

[36]  E. D. Hanson,et al.  Morphological, biological, and ecological considerations in the phylogeny of parasitic nematodes. , 1963 .