Construction of a chromosome specific library of human MARs and mapping of matrix attachment regions on human chromosome 19.

Using a novel procedure a representative human chromosome 19-specific library was constructed of short sequences, which bind preferentially to the nuclear matrix (matrix attachment regions, or MARs). Judging by 20 clones sequenced so far, the library contains > 50% of human inserts, about 90% of which are matrix-binding by the in vitro test. Computer analysis of sequences of eight human MARs did not reveal any significant homologies with the EMBL Nucleotide Data Base entries as well as between MARs themselves. Eight MARs were assigned to individual positions on the chromosome 19 physical map. The library constructed can serve as a good source of MAR sequences for comparative analysis and classification and for further chromosome mapping of MARs as well.

[1]  Tom Slezak,et al.  An integrated metric physical map of human chromosome 19 , 1995, Nature Genetics.

[2]  T. Boulikas,et al.  Nature of DNA sequences at the attachment regions of genes to the nuclear matrix , 1993, Journal of cellular biochemistry.

[3]  Susan M. Gasser,et al.  A glimpse at chromosomal order , 1987 .

[4]  V A McKusick,et al.  Current trends in mapping human genes , 1991, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[5]  K. Pienta,et al.  The tissue matrix: cell dynamics and hormone action. , 1990, Endocrine reviews.

[6]  S. Volik,et al.  An arrayed library enriched in hncDNA corresponding to transcribed sequences of human chromosome 19: preparation and analysis. , 1995, Genetic analysis : biomolecular engineering.

[7]  P. Cockerill,et al.  Chromosomal loop anchorage of the kappa immunoglobulin gene occurs next to the enhancer in a region containing topoisomerase II sites , 1986, Cell.

[8]  J. Bode,et al.  Biological significance of unwinding capability of nuclear matrix-associating DNAs. , 1992, Science.

[9]  E. Myers,et al.  Basic local alignment search tool. , 1990, Journal of molecular biology.

[10]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[11]  D. Galas,et al.  A new five-year plan for the U.S. Human Genome Project. , 1993, Science.

[12]  A. Kerlavage,et al.  Complementary DNA sequencing: expressed sequence tags and human genome project , 1991, Science.

[13]  J. Craig Venter,et al.  3,400 new expressed sequence tags identify diversity of transcripts in human brain , 1993, Nature Genetics.

[14]  M. Ashburner.  A Laboratory manual , 1989 .

[15]  B. Trask,et al.  A new system for high-resolution DNA sequence mapping interphase pronuclei. , 1991, Genomics.

[16]  U. K. Laemmli,et al.  Organization of the higher-order chromatin loop: specific DNA attachment sites on nuclear scaffold , 1984, Cell.

[17]  T. Boulikas,et al.  Multitude of inverted repeats characterizes a class of anchorage sites of chromatin loops to the nuclear matrix , 1993, Journal of cellular biochemistry.

[18]  U. Hochgeschwender.  Toward a transcriptional map of the human genome. , 1992, Trends in genetics : TIG.

[19]  P. D. de Jong,et al.  Constructing chromosome- and region-specific cosmid maps of the human genome. , 1989, Genome.

[20]  L. Phi-Van,et al.  Association of DNA with nuclear matrix , 1990 .

[21]  M. Adams,et al.  Chromosomal distribution of 320 genes from a brain cDNA library , 1993, Nature Genetics.

[22]  S. Volik,et al.  Mapping of transcribed sequences on human chromosome 19. , 1995, DNA sequence : the journal of DNA sequencing and mapping.

[23]  G. Gyapay,et al.  A second-generation linkage map of the human genome , 1992, Nature.

[24]  D. Jackson,et al.  The attachments of chromatin loops to the nucleoskeleton. , 1992, Cell biology international reports.

[25]  M. Adams,et al.  Chromosomal assignment of 46 brain cDNAs. , 1992, Genomics.

[26]  C. Cantor,et al.  New techniques for physical mapping of the human genome , 1991, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[27]  U. K. Laemmli,et al.  Interaction of DNA with nuclear scaffolds in vitro. , 1988, Journal of molecular biology.