Natural developmental variations in leaf and plant senescence in Arabidopsis thaliana.

Leaf senescence is a developmentally regulated process that contributes to nutrient redistribution during reproductive growth and finally leads to tissue death. Manipulating leaf senescence through breeding or genetic engineering may help to improve important agronomic traits, such as crop yield and the storage life of harvested organs. Here, we studied natural variations in the regulation of plant senescence among 16 Arabidopsis thaliana accessions. Chlorophyll content and the proportion of yellow leaves were used as indicator parameters to determine leaf and plant senescence respectively. Our study indicated significant genotype effects on the onset and development of senescence. We selected three late- and five early-senescence accessions for further physiological studies. The relationship between leaf and plant senescence was accession-dependent. There was a significant correlation between plant senescence and the total number of leaves, siliques and plant bolting age. We monitored expression of two senescence marker genes, SAG12 and WRKY53, to evaluate progression of senescence. Our data revealed that chlorophyll content does not fully reflect leaf age, because even fully green leaves had already commenced senescence at the molecular level. Integrating senescence parameters, such as the proportion of senescent leaves, at the whole plant level provided a better indication of the molecular status of the plant than single leaf senescence parameters.

[1]  B. Mueller‐Roeber,et al.  Transcription factors regulating leaf senescence in Arabidopsis thaliana. , 2008, Plant biology.

[2]  L. Taconnat,et al.  Genetic Variation Suggests Interaction between Cold Acclimation and Metabolic Regulation of Leaf Senescence1[W][OA] , 2006, Plant Physiology.

[3]  S. Hörtensteiner.  Chlorophyll degradation during senescence. , 2006, Annual review of plant biology.

[4]  U. Flügge,et al.  Transcription Analysis of Arabidopsis Membrane Transporters and Hormone Pathways during Developmental and Induced Leaf Senescence1[W] , 2006, Plant Physiology.

[5]  E. Pelzer,et al.  Effect of sugar-induced senescence on gene expression and implications for the regulation of senescence in Arabidopsis , 2006, Planta.

[6]  J. Guiamet,et al.  Quantitative trait loci analysis of leaf and plant longevity in Arabidopsis thaliana. , 2006, Journal of experimental botany.

[7]  Johannes Müller,et al.  Successive maturation and senescence of individual leaves during barley whole plant ontogeny reveals temporal and spatial regulation of photosynthetic function in conjunction with C and N metabolism. , 2005, Journal of plant physiology.

[8]  C. Masclaux-Daubresse,et al.  Characterization of Markers to Determine the Extent and Variability of Leaf Senescence in Arabidopsis. A Metabolic Profiling Approach1 , 2005, Plant Physiology.

[9]  Vicky Buchanan-Wollaston,et al.  Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. , 2005, The Plant journal : for cell and molecular biology.

[10]  A. Wingler,et al.  Natural variation in the regulation of leaf senescence and relation to other traits in Arabidopsis , 2005 .

[11]  H. Nam,et al.  The delayed leaf senescence mutants of Arabidopsis, ore1, ore3, and ore9 are tolerant to oxidative stress. , 2004, Plant & cell physiology.

[12]  A. Wingler,et al.  Interactions of abscisic acid and sugar signalling in the regulation of leaf senescence , 2004, Planta.

[13]  Mark Stitt,et al.  Real-time RT-PCR profiling of over 1400 Arabidopsis transcription factors: unprecedented sensitivity reveals novel root- and shoot-specific genes. , 2004, The Plant journal : for cell and molecular biology.

[14]  I. Gut,et al.  Nested core collections maximizing genetic diversity in Arabidopsis thaliana. , 2004, The Plant journal : for cell and molecular biology.

[15]  A. Moorman,et al.  Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data , 2003, Neuroscience Letters.

[16]  J. Lundeberg,et al.  Gene Expression in Autumn Leaves1 , 2003, Plant Physiology.

[17]  E. Harrison,et al.  The molecular analysis of leaf senescence--a genomics approach. , 2002, Plant biotechnology journal.

[18]  Stefan Hörtensteiner,et al.  Nitrogen metabolism and remobilization during senescence. , 2002, Journal of experimental botany.

[19]  R. Amasino,et al.  Senescence is induced in individually darkened Arabidopsis leaves, but inhibited in whole darkened plants. , 2001, Plant physiology.

[20]  K. Hinderhofer,et al.  Identification of a transcription factor specifically expressed at the onset of leaf senescence , 2001, Planta.

[21]  U. Sonnewald,et al.  High CO2‐mediated down‐regulation of photosynthetic gene transcripts is caused by accelerated leaf senescence rather than sugar accumulation , 2000, FEBS letters.

[22]  R. Amasino,et al.  Molecular aspects of leaf senescence. , 2000, Trends in plant science.

[23]  H. Thomas,et al.  Five ways to stay green. , 2000, Journal of experimental botany.

[24]  R. Amasino,et al.  Identification of a promoter region responsible for the senescence-specific expression of SAG12 , 1999, Plant Molecular Biology.

[25]  J. Miller,et al.  Senescence-associated gene expression during ozone-induced leaf senescence in Arabidopsis. , 1999, Plant physiology.

[26]  H. Nam,et al.  Differential expression of senescence-associated mRNAs during leaf senescence induced by different senescence-inducing factors in Arabidopsis , 1998, Plant Molecular Biology.

[27]  R. Amasino,et al.  A comparison of the expression patterns of several senescence-associated genes in response to stress and hormone treatment , 1998, Plant Molecular Biology.

[28]  U. Feller,et al.  Influence of nitrogen deficiency on senescence and the amounts of RNA and proteins in wheat leaves , 1998 .

[29]  P. Trivedi,et al.  Expression of chloroplastic genes during autumnal senescence in a deciduous tree Populus deltiodes , 1997, Biochemistry and molecular biology international.

[30]  H. Nam,et al.  Identification of three genetic loci controlling leaf senescence in Arabidopsis thaliana , 1997 .

[31]  Nam,et al.  The molecular genetic analysis of leaf senescence. , 1997, Current opinion in biotechnology.

[32]  Vicky Buchanan-Wollaston,et al.  The molecular biology of leaf senescence , 1997 .

[33]  R. Amasino,et al.  Making Sense of Senescence (Molecular Genetic Regulation and Manipulation of Leaf Senescence) , 1997, Plant physiology.

[34]  H. Thomas,et al.  Chlorophyll Breakdown in Senescent Leaves , 1996, Plant physiology.

[35]  A. Bleecker,et al.  Ethylene regulates the timing of leaf senescence in Arabidopsis , 1995 .

[36]  H. Thomas,et al.  The timing of maize leaf senescence and characterisation of senescence-related cDNAs , 1995 .

[37]  A. Bleecker,et al.  Developmental and age-related processes that influence the longevity and senescence of photosynthetic tissues in arabidopsis. , 1993, The Plant cell.

[38]  A. Wingler,et al.  The role of sugars in integrating environmental signals during the regulation of leaf senescence. , 2006, Journal of experimental botany.

[39]  Stephen J. Tonsor,et al.  Gene function beyond the single trait: natural variation, gene effects, and evolutionary ecology in Arabidopsis thaliana , 2005 .

[40]  J. Falk,et al.  Genetic, Metabolic and Environmental Factors Associated with Aging in Plants , 2003 .

[41]  R. Amasino,et al.  Nutrients mobilized from leaves of Arabidopsis thaliana during leaf senescence , 2001 .

[42]  M Koornneef,et al.  Naturally occurring variation in Arabidopsis: an underexploited resource for plant genetics. , 2000, Trends in plant science.

[43]  O. Monje,et al.  Inherent limitations of nondestructive chlorophyll meters: a comparison of two types of meters. , 1992, HortScience : a publication of the American Society for Horticultural Science.