Milling pretreatment of sugarcane bagasse and straw for enzymatic hydrolysis and ethanol fermentation.

The effectiveness of ball milling (BM) and wet disk milling (WDM) on treating sugarcane bagasse and straw were compared. Pretreated materials were characterized by wide angle X-ray diffraction analysis, particle-size distribution and scanning electron microscopy and the effectiveness of pretreatments was evaluated by enzymatic hydrolysis and fermentation. Glucose and xylose hydrolysis yields at optimum conditions for BM-treated bagasse and straw were 78.7% and 72.1% and 77.6% and 56.8%, respectively. Maximum glucose and xylose yields for bagasse and straw using WDM were 49.3% and 36.7% and 68.0% and 44.9%, respectively. BM improved the enzymatic hydrolysis by decreasing the crystallinity, while the defibrillation effect observed for WDM samples seems to have favored enzymatic conversion. Bagasse and straw BM hydrolysates were fermented by Saccharomyces cerevisiae strains. Ethanol yields from total fermentable sugars using a C6-fermenting strain reached 89.8% and 91.8% for bagasse and straw hydrolysates, respectively, and 82% and 78% when using a C6/C5 fermenting strain.

[1]  Kassiap Deepchand.  Characteristics, present use and potential of sugar cane tops and leaves , 1986 .

[2]  S. Sawayama,et al.  Combining hot-compressed water and ball milling pretreatments to improve the efficiency of the enzymatic hydrolysis of eucalyptus , 2008, Biotechnology for biofuels.

[3]  G. Zacchi,et al.  The generation of fermentation inhibitors during dilute acid hydrolysis of softwood , 1999 .

[4]  L. Lynd,et al.  A comparison of liquid hot water and steam pretreatments of sugar cane bagasse for bioconversion to ethanol. , 2002, Bioresource technology.

[5]  T. K. Ghose.  Measurement of cellulase activities , 1987 .

[6]  M. Galbe,et al.  Bio-ethanol--the fuel of tomorrow from the residues of today. , 2006, Trends in biotechnology.

[7]  B. Saha,et al.  Dilute acid pretreatment, enzymatic saccharification and fermentation of wheat straw to ethanol , 2005 .

[8]  L. Segal',et al.  An Empirical Method for Estimating the Degree of Crystallinity of Native Cellulose Using the X-Ray Diffractometer , 1959 .

[9]  O. Lucon,et al.  Brazilian sugarcane ethanol: lessons learned , 2006 .

[10]  M. Galbe,et al.  Ethanol production from enzymatic hydrolysates of sugarcane bagasse using recombinant xylose-utilising Saccharomyces cerevisiae , 2002 .

[11]  N. Meinander,et al.  Main and interaction effects of acetic acid, furfural, and p-hydroxybenzoic acid on growth and ethanol productivity of yeasts. , 1999, Biotechnology and bioengineering.

[12]  Akira Yabe,et al.  System Efficiency and Economical Analysis of System for Producing Energy Material from Wooden Biomass , 2006 .

[13]  Mats Galbe,et al.  Optimisation of steam pretreatment of SO2-impregnated mixed softwoods for ethanol production , 1998 .

[14]  M. J. Effland,et al.  Influence of Fine Grinding on the Hydrolysis of Cellulosic Materials—Acid Vs. Enzymatic , 1979 .

[15]  Shigeki Sawayama,et al.  Bioethanol production performance of five recombinant strains of laboratory and industrial xylose-fermenting Saccharomyces cerevisiae. , 2009, Bioresource technology.

[16]  Shinji Fujimoto,et al.  Wet disk milling pretreatment without sulfuric acid for enzymatic hydrolysis of rice straw. , 2009, Bioresource technology.

[17]  Seungdo Kim,et al.  Recent process improvements for the ammonia fiber expansion (AFEX) process and resulting reductions in minimum ethanol selling price. , 2008, Bioresource technology.

[18]  Anne Belinda Thomsen,et al.  Wet oxidation as a pretreatment method for enhancing the enzymatic convertibility of sugarcane bagasse , 2007 .

[19]  Dehua Liu,et al.  Peracetic acid pretreatment of sugarcane bagasse for enzymatic hydrolysis: a continued work , 2008 .

[20]  A. Hira,et al.  No substitute for oil? How Brazil developed its ethanol industry , 2009 .

[21]  A. Isogai,et al.  CRYSTALLINITY INDEXES OF CELLULOSIC MATERIALS , 1990 .

[22]  M. Taherzadeh,et al.  Pretreatment of Lignocellulosic Wastes to Improve Ethanol and Biogas Production: A Review , 2008, International journal of molecular sciences.

[23]  D. Ryu,et al.  Energy requirements and process design considerations in compression‐milling pretreatment of cellulosic wastes for enzymatic hydrolysis , 1980 .

[24]  N. Tanaka,et al.  Pretreatment of eucalyptus wood chips for enzymatic saccharification using combined sulfuric acid‐free ethanol cooking and ball milling , 2008, Biotechnology and bioengineering.

[25]  C. M. Kinoshita,et al.  Steam explosion of sugarcane bagasse as a pretreatment for conversion to ethanol. , 1998 .

[26]  Andrew J. McAloon,et al.  Understanding the reductions in US corn ethanol production costs: an experience curve approach , 2009 .

[27]  Luis Augusto Barbosa Cortez,et al.  Can Brazil replace 5% of the 2025 gasoline world demand with ethanol? , 2009 .

[28]  J. Saddler,et al.  Enhancing the enzymatic hydrolysis of cellulosic materials using simultaneous ball milling. , 2002, Applied biochemistry and biotechnology.

[29]  R. Slade,et al.  New improvements for lignocellulosic ethanol. , 2009, Current opinion in biotechnology.