Detection of megalocytivirus from imported tropical ornamental fish, paradise fish Macropodus opercularis.

Megalocytivirus was detected from paradise fish Macropodus opercularis imported from Indonesia. Four of 11 fish (36%) in 2006 and 40 of 117 fish (34%) in 2008 were found to be PCR-positive for megalocytivirus. Phylogenetic analysis based on partial major capsid protein (MCP) gene nucleotide sequences revealed that the sequences detected in paradise fish were classified as Genotype II, which includes freshwater fish isolates from Southeast Asian countries, closely related to infectious spleen and kidney necrosis virus (ISKNV), Murray cod iridovirus (MCIV), and dwarf gourami iridovirus (DGIV-2004). Paradise fish was added as a new host for megalocytivirus based on this study.

[1]  H. Jeong,et al.  Transmission of iridovirus from freshwater ornamental fish (pearl gourami) to marine fish (rock bream). , 2008, Diseases of aquatic organisms.

[2]  S. Jung,et al.  Genetic variation and geographic distribution of megalocytiviruses , 2008, The Journal of Microbiology.

[3]  Joong-Kyun Kim,et al.  Outbreaks and risks of infectious spleen and kidney necrosis virus disease in freshwater ornamental fishes. , 2008, Diseases of aquatic organisms.

[4]  R. Whittington,et al.  Global trade in ornamental fish from an Australian perspective: the case for revised import risk analysis and management strategies. , 2007, Preventive veterinary medicine.

[5]  M. Nei,et al.  MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. , 2007, Molecular biology and evolution.

[6]  R. Whittington,et al.  Experimental transmission and virulence of a megalocytivirus (Family Iridoviridae) of dwarf gourami (Colisa lalia) from Asia in Murray cod (Maccullochella peelii peelii) in Australia , 2006 .

[7]  R. Whittington,et al.  The molecular epidemiology of iridovirus in Murray cod (Maccullochella peelii peelii) and dwarf gourami (Colisa lalia) from distant biogeographical regions suggests a link between trade in ornamental fish and emerging iridoviral diseases. , 2006, Molecular and cellular probes.

[8]  S. Jung,et al.  Characterization of an iridovirus detected from cultured turbot Scophthalmus maximus in Korea. , 2005, Diseases of aquatic organisms.

[9]  M. A. Park,et al.  Sequence variation in the gene encoding the major capsid protein of Korean fish iridoviruses , 2005, Archives of Virology.

[10]  T. Miyazaki,et al.  Iridovirus disease in two ornamental tropical freshwater fishes: African lampeye and dwarf gourami. , 2002, Diseases of aquatic organisms.

[11]  T. Miyazaki,et al.  Viral DNA sequences of genes encoding the ATPase and the major capsid protein of tropical iridovirus isolates which are pathogenic to fishes in Japan, South China Sea and Southeast Asian countries , 2002, Archives of Virology.

[12]  S. Jung,et al.  Iridovirus‐like infection associated with high mortalities of striped beakperch, Oplegnathus fasciatus (Temminck et Schlegel), in southern coastal areas of the Korean peninsula , 2000 .

[13]  S. Chan,et al.  Systemic disease caused by an iridovirus‐like agent in cultured mandarinfish, Siniperca chuatsi (Basilewsky), in China , 2000 .

[14]  J. Thompson,et al.  The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. , 1997, Nucleic acids research.

[15]  K. Nakajima,et al.  Iridovirus infection of cultured red sea bream, Pagrus major , 1992 .

[16]  S. Jung,et al.  Detection of Red Sea Bream Iridovirus (RSIV) from marine fish in the Southern Coastal Area and East China Sea , 2007 .

[17]  오명주,et al.  Detection of Red Sea Bream Iridovirus (RSIV) from marine fish in the Southern Coastal Area and East China Sea , 2007 .

[18]  L. Lü,et al.  Molecular epidemiology and phylogenetic analysis of a marine fish infectious spleen and kidney necrosis virus-like (ISKNV-like) virus , 2006, Archives of Virology.

[19]  H. Jeong,et al.  Detection and distribution of iridoviruses in five freshwater ornamental fish species , 2006 .

[20]  S. Jung,et al.  Detection of RSIV (Red Sea Bream Iridovirus) in the Cultured Marine Fish by the Polymerase Chain Reaction , 1999 .