Enzyme‐Based Multi‐Component Optical Nanoprobes for Sequence‐ Specific Detection of DNA Hybridization

Seeing is believing. A novel multicomponent gold nanoparticle-based nanoprobe is assembled with a thiolated DNA detection probe, horseradish peroxidase (HRP), and bovine serum albumin (BSA). In this nanoprobe, the DNA detection probe is used to construct complexes for "sandwich" -based DNA detection, HRP translates this hybridization event into an enzymatic catalysis-based optical signal, while and BSA acts as a non-specific blocker.

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