Teriparatide with octacalcium phosphate collagen composite stimulates osteogenic factors.

Octacalcium phosphate and collagen composite (OCPcol) promotes osteogenic differentiation and angiogenesis, thereby enhancing bone regeneration. Although a newly developed freeze-dried composite of OCPcol and teriparatide (OCPcolTPTD) reinforced bone regeneration more than OCPcol, the mechanism of bone regeneration remains unresolved. Here, disks containing OCPcolTPTD, OCPcol, or β-tricalcium phosphate (β-TCP) col were inserted into rodents with calvarial bone defects, before euthanasia 4 weeks later. Immunohistochemical and histochemical analyses were performed on bone samples to evaluate bone matrix development, angiogenesis, and osteoclast and osteoblast localization. In the OCPcolTPTD and OCPcol groups, bone regeneration was observed at the surface of calvarial dura mater and around acidophilic granular cells with abundant collagenous fiber-containing cells. Furthermore, the newly formed bone in the OCPcolTPTD group showed a larger total area and individual separated area than the other groups. Various osteogenic proteins were detected in the regenerated bone and peri-bone tissues via histochemistry and immunohistochemistry. Although the expression of several osteogenic biomarkers in the OCPcolTPTD group after 4 weeks of implantation was significantly lower than that in the OCPcol group, new bone formation by OCPcolTPTD in the center of the defect, where bone regeneration is difficult, tended to be superior to that by OCPcol. These results suggest that OCPcolTPTD enhanced bone regeneration more evenly and homogenously than OCPcol.

[1]  T. Kawai,et al.  Clinical study of octacalcium phosphate and collagen composite in oral and maxillofacial surgery , 2020, Journal of tissue engineering.

[2]  T. Kawai,et al.  Octacalcium phosphate collagen composite stimulates the expression and activity of osteogenic factors to promote bone regeneration , 2019, Journal of tissue engineering and regenerative medicine.

[3]  Lei Lu,et al.  Insulin-like growth factor-1 engaged in the mandibular condylar cartilage degeneration induced by experimental unilateral anterior crossbite. , 2019, Archives of oral biology.

[4]  T. Kawai,et al.  Single‐dose local administration of teriparatide with a octacalcium phosphate collagen composite enhances bone regeneration in a rodent critical‐sized calvarial defect , 2017, Journal of biomedical materials research. Part B, Applied biomaterials.

[5]  T. Kawai,et al.  Octacalcium phosphate collagen composite facilitates bone regeneration of large mandibular bone defect in humans , 2017, Journal of tissue engineering and regenerative medicine.

[6]  E. Hochuli-Vieira,et al.  Use of autogenous bone and beta-tricalcium phosphate in maxillary sinus lifting: histomorphometric study and immunohistochemical assessment of RUNX2 and VEGF. , 2017, International journal of oral and maxillofacial surgery.

[7]  T. Kawai,et al.  Clinical safety and efficacy of implantation of octacalcium phosphate collagen composites in tooth extraction sockets and cyst holes , 2016, Journal of tissue engineering.

[8]  Pamela Habibovic,et al.  Calcium phosphates in biomedical applications: materials for the future? , 2016 .

[9]  D. Rowe,et al.  Combination therapy with PTH and DBM cannot heal a critical sized murine femoral defect , 2015, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[10]  T. Kawai,et al.  The Regenerated Bone Quality by Implantation of Octacalcium Phosphate Collagen Composites in a Canine Alveolar Cleft Model , 2014, The Cleft palate-craniofacial journal : official publication of the American Cleft Palate-Craniofacial Association.

[11]  C. Susin,et al.  Effect of bone morphogenetic protein-2, demineralized bone matrix and systemic parathyroid hormone (1-34) on local bone formation in a rat calvaria critical-size defect model. , 2013, Journal of periodontal research.

[12]  L. Mccauley,et al.  Parathyroid Hormone Applications in the Craniofacial Skeleton , 2013, Journal of dental research.

[13]  T. Kawai,et al.  Reconstruction of critical-sized bone defect in dog skull by octacalcium phosphate combined with collagen. , 2011, Clinical implant dentistry and related research.

[14]  D. Baur,et al.  Effect of intermittent systemic administration of recombinant parathyroid hormone (1-34) on mandibular fracture healing in rats. , 2010, Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons.

[15]  G. Karsenty,et al.  Genetic control of bone formation. , 2009, Annual review of cell and developmental biology.

[16]  T. Anada,et al.  The primacy of octacalcium phosphate collagen composites in bone regeneration. , 2007, Journal of biomedical materials research. Part A.

[17]  A Tallgren,et al.  The continuing reduction of the residual alveolar ridges in complete denture wearers: a mixed-longitudinal study covering 25 years. , 2003, The Journal of prosthetic dentistry.

[18]  Y. Soini,et al.  Endothelial nitric oxide synthase is strongly expressed in malignant mesothelioma but does not associate with vascular density or the expression of VEGF, FLK1 or FLT1 , 2001, Histopathology.

[19]  O. Suzuki,et al.  Bone formation on synthetic precursors of hydroxyapatite. , 1991, The Tohoku journal of experimental medicine.

[20]  R. Sauer,et al.  The amino-acid sequence of the amino-terminal 37 residues of human parathyroid hormone. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[21]  A. Kamali,et al.  Synergistic effect of strontium, bioactive glass and nano-hydroxyapatite promotes bone regeneration of critical-sized radial bone defects. , 2019, Journal of biomedical materials research. Part B, Applied biomaterials.

[22]  I. Asahina,et al.  First clinical application of octacalcium phosphate collagen composite on bone regeneration in maxillary sinus floor augmentation: A prospective, single-arm, open-label clinical trial. , 2019, Journal of biomedical materials research. Part B, Applied biomaterials.

[23]  T. Kawai,et al.  Comparison of bone regeneration between octacalcium phosphate/collagen composite and β-tricalcium phosphate in canine calvarial defect. , 2013, Oral surgery, oral medicine, oral pathology and oral radiology.

[24]  O. Suzuki,et al.  Implanted octacalcium phosphate is more resorbable than beta-tricalcium phosphate and hydroxyapatite. , 2002, Journal of biomedical materials research.

[25]  O. Suzuki,et al.  Multinucleated giant cells recruited by implantation of octacalcium phosphate (OCP) in rat bone marrow share ultrastructural characteristics with osteoclasts. , 1997, Journal of electron microscopy.