A novel series of new eleven benzocaine hydrazide derivatives, N-(4-{[2-(nonsubstituted/ substitutedfuryl/ phenyl/ pyridinyl/ thienyl/ pyrrole)methylidene]hydrazinyl] carbonyl}phenyl) benzamides [3a-k] have been synthesized in this study. The structures of the new compounds were determined by spectral (FT-IR and 1H-NMR) methods and their purity was proven by elemental analysis and thin layer chromatography. These compounds were evaluated for in vitro antibacterial activity by using micro-well dilution method against Escherichia coli ATCC 10536, Escherichia coli ATCC 15442, Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 15442, Acinetobacter baumannii , Klebsiella pneumonia ATCC 13883.