Purpose: To evaluate discrepancies in Goldmann tonometer readings in eyes with varying degrees of corneal hydration and stromal amount. Methods. 6 Eye Bank eyes, donated by Florida Lion's Eye Bank, were evaluated. Each eye was affixed to a customized artificial orbit system with intraocular pressure (IOP) measured directly by a pressure transducer inserted into the vitreous and with a Goldmann tonometric readings. The eyes were dehydrated for 5-minute intervals in a 30% Dextran-BSS solution, with readings taken between each submersion. Once corneal thickness stabilized, a corneal trephination of 6mm was made. The corneal buttons were frozen and dehydrated by lyophilization and weighed. Results. Preliminary results show a possible overestimation in thicker corneas and an underestimation in thinner corneas, as previously published. Corresponding data on the weight of corneal material is provided. Further studies need to be conducted to determine statistical significance of the data. Conclusion. This study uses Eye Bank eyes with a protocol that produces results similar to previously published results. Further studies in correlating the amount of corneal stroma and discrepancies in Goldmann tonometer readings of intraocular pressure are important especially with the increasing acceptance of corneal refractive surgeries.
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