A poly(D,L-lactide) resin for the preparation of tissue engineering scaffolds by stereolithography.

Porous polylactide constructs were prepared by stereolithography, for the first time without the use of reactive diluents. Star-shaped poly(D,L-lactide) oligomers with 2, 3 and 6 arms were synthesised, end-functionalised with methacryloyl chloride and photo-crosslinked in the presence of ethyl lactate as a non-reactive diluent. The molecular weights of the arms of the macromers were 0.2, 0.6, 1.1 and 5 kg/mol, allowing variation of the crosslink density of the resulting networks. Networks prepared from macromers of which the molecular weight per arm was 0.6 kg/mol or higher had good mechanical properties, similar to linear high-molecular weight poly(D,L-lactide). A resin based on a 2-armed poly(D,L-lactide) macromer with a molecular weight of 0.6 kg/mol per arm (75 wt%), ethyl lactate (19 wt%), photo-initiator (6 wt%), inhibitor and dye was prepared. Using this resin, films and computer-designed porous constructs were accurately fabricated by stereolithography. Pre-osteoblasts showed good adherence to these photo-crosslinked networks. The proliferation rate on these materials was comparable to that on high-molecular weight poly(D,L-lactide) and tissue culture polystyrene.

[1]  Jeffrey A. Hubbell,et al.  Bioerodible hydrogels based on photopolymerized poly(ethylene glycol)-co-poly(.alpha.-hydroxy acid) diacrylate macromers , 1993 .

[2]  Robert Weiss,et al.  Morphological Control in Multiphase Polymer Mixtures , 1996 .

[3]  P Rüegsegger,et al.  3D Micro-Tomographic imaging and Quantitative Morphometry for the Nondestructive Evaluation of Porous Biomaterials , 1996 .

[4]  K. Shakesheff,et al.  The effect of anisotropic architecture on cell and tissue infiltration into tissue engineering scaffolds. , 2006, Biomaterials.

[5]  Thierry Chartier,et al.  Ceramic suspensions suitable for stereolithography , 1998 .

[6]  Richard Tuli,et al.  Human mesenchymal progenitor cell-based tissue engineering of a single-unit osteochondral construct. , 2004, Tissue engineering.

[7]  Paul N Manson,et al.  Variable cytocompatibility of six cell lines with photoinitiators used for polymerizing hydrogels and cell encapsulation. , 2005, Biomaterials.

[8]  R. Langer,et al.  Wetting of poly(L-lactic acid) and poly(DL-lactic-co-glycolic acid) foams for tissue culture. , 1994, Biomaterials.

[9]  Krishnendu Roy,et al.  A digital micro-mirror device-based system for the microfabrication of complex, spatially patterned tissue engineering scaffolds. , 2006, Journal of biomedical materials research. Part A.

[10]  R. Storey,et al.  Synthesis of bioabsorbable networks from methacrylate-endcapped polyesters , 1993 .

[11]  R. Harris,et al.  Layer Manufacturing for in Vivo Devices , 2006, Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine.

[12]  Paul F. Jacobs,et al.  Rapid Prototyping & Manufacturing: Fundamentals of Stereolithography , 1992 .

[13]  S J Bryant,et al.  Cytocompatibility of UV and visible light photoinitiating systems on cultured NIH/3T3 fibroblasts in vitro , 2000, Journal of biomaterials science. Polymer edition.

[14]  C. Bowen,et al.  Development of Modelling Methods for Materials to be Used as Bone Substitutes , 2007 .

[15]  Dong-Woo Cho,et al.  Application of microstereolithography in the development of three-dimensional cartilage regeneration scaffolds , 2008, Biomedical microdevices.

[16]  Michael J Yaszemski,et al.  Poly(propylene fumarate) bone tissue engineering scaffold fabrication using stereolithography: effects of resin formulations and laser parameters. , 2007, Biomacromolecules.

[17]  Manabu Mizutani,et al.  Liquid acrylate-endcapped biodegradable poly(epsilon-caprolactone-co-trimethylene carbonate). II. Computer-aided stereolithographic microarchitectural surface photoconstructs. , 2002, Journal of biomedical materials research.

[18]  J. Fitzgerald,et al.  Vitrification and curing studies of a photopolymerizable semi‐interpenetrating polymer network. II , 1990 .

[19]  Arjan Vissink,et al.  Resorbable screws for fixation of autologous bone grafts. , 2006, Clinical oral implants research.

[20]  M. B. Claase,et al.  Enhanced bone marrow stromal cell adhesion and growth on segmented poly(ether ester)s based on poly(ethylene oxide) and poly(butylene terephthalate). , 2003, Biomacromolecules.

[21]  Malcolm N. Cooke,et al.  Use of stereolithography to manufacture critical-sized 3D biodegradable scaffolds for bone ingrowth. , 2003, Journal of biomedical materials research. Part B, Applied biomaterials.

[22]  K. Leong,et al.  The design of scaffolds for use in tissue engineering. Part I. Traditional factors. , 2001, Tissue engineering.

[23]  E. Stelnicki,et al.  Use of Absorbable Poly (D,L) Lactic Acid Plates in Cranial-Vault Remodeling: Presentation of the First Case and Lessons Learned about Its Use , 2005, The Cleft palate-craniofacial journal : official publication of the American Cleft Palate-Craniofacial Association.

[24]  J. Seppälä,et al.  Structure modification and crosslinking of methacrylated polylactide oligomers , 2002 .

[25]  Thomas Boland,et al.  Rapid prototyping of tissue-engineering constructs, using photopolymerizable hydrogels and stereolithography. , 2004, Tissue engineering.

[26]  Ryan B. Wicker,et al.  Stereolithography of Three-Dimensional Bioactive Poly(Ethylene Glycol) Constructs with Encapsulated Cells , 2006, Annals of Biomedical Engineering.

[27]  K. Dušek,et al.  Features of network formation in the chain crosslinking (co)polymerization , 1980 .

[28]  Krishnendu Roy,et al.  Laser-layered microfabrication of spatially patterned functionalized tissue-engineering scaffolds. , 2005, Journal of biomedical materials research. Part B, Applied biomaterials.

[29]  Jan Feijen,et al.  Fumaric acid monoethyl ester-functionalized poly(D,L-lactide)/N-vinyl-2-pyrrolidone resins for the preparation of tissue engineering scaffolds by stereolithography. , 2009, Biomacromolecules.

[30]  J. Feijen,et al.  Preparation of biodegradable networks by photo-crosslinking lactide, epsilon-caprolactone and trimethylene carbonate-based oligomers functionalized with fumaric acid monoethyl ester. , 2005, Biomaterials.

[31]  S. Hollister.  Porous scaffold design for tissue engineering , 2005, Nature materials.