A rapid reconstruction algorithm for three-dimensional scanning images

Abstract A `simulated fluorescence' three-dimensional reconstruction algorithm, which is especially suitable for confocal images of partial transparent biological samples, is proposed in this paper. To make the retina projection of the object reappear and to avoid excessive memory consumption, the original image is rotated and compressed before the processing. A left image and a right image are mixed by different colors to increase the sense of stereo. The details originally hidden in deep layers are well exhibited with the aid of an `auxiliary directional source'. In addition, the time consumption is greatly reduced compared with conventional methods such as `ray tracing'. The realization of the algorithm is interpreted by a group of reconstructed images.