A portable fluorescent sensing system using multiple LEDs

This paper presents a portable fluorescent sensing system that utilizes different light emitting diode (LED) excitation lights for multiple target detection. In order to identify different analytes, three different wavelengths (385 nm, 448 nm, and 590 nm) of excitation light emitting diodes were used to selectively stimulate the target analytes. A highly sensitive silicon photomultiplier (SiPM) was used to detect corresponding fluorescent signals from each analyte. Based on the unique fluorescent response of each analyte, it is possible to simultaneously differentiate one analyte from the other in a mixture of target analytes. A portable system was designed and fabricated consisting of a display module, battery, data storage card, and sample loading tray into a compact 3D-printed jig. The portable sensor system was demonstrated for quantification and differentiation of microalgae (Chlorella vulgaris) and cyanobacteria (Spirulina) by measuring fluorescent responses of chlorophyll a in microalgae and phycocyanin in cyanobacteria. Obtained results suggest that the developed portable sensor system could be used as a generic fluorescence sensor platform for on-site detection of multiple analytes of interest.

[1]  F. Figueroa,et al.  Use of in vivo chlorophyll fluorescence to estimate photosynthetic activity and biomass productivity in microalgae grown in different culture systems , 2013 .

[2]  Alan Weidemann,et al.  Phytoplankton spectral absorption as influenced by community size structure and pigment composition , 2003 .

[3]  Luke P. Lee,et al.  Systematic characterization of degas-driven flow for poly(dimethylsiloxane) microfluidic devices. , 2011, Biomicrofluidics.

[4]  Samuel K Sia,et al.  An integrated approach to a portable and low-cost immunoassay for resource-poor settings. , 2004, Angewandte Chemie.

[5]  E. Gantt,et al.  Pigment protein complexes and the concept of the photosynthetic unit: Chlorophyll complexes and phycobilisomes , 1996, Photosynthesis Research.

[6]  Andreas Holzinger,et al.  Algae and UV irradiation: effects on ultrastructure and related metabolic functions. , 2006, Micron.

[7]  Ali Khademhosseini,et al.  Nano/Microfluidics for diagnosis of infectious diseases in developing countries. , 2010, Advanced drug delivery reviews.

[8]  Jin-Woo Choi,et al.  Disposable smart lab on a chip for point-of-care clinical diagnostics , 2004, Proceedings of the IEEE.

[9]  Yi Lu,et al.  Portable and quantitative detection of protein biomarkers and small molecular toxins using antibodies and ubiquitous personal glucose meters. , 2012, Analytical chemistry.

[10]  M. Canle,et al.  Environmental Applications of Excitation-Emission Spectrofluorimetry: An In-Depth Review I , 2013 .

[11]  Jin-Woo Choi,et al.  A portable fluorescent sensor for on-site detection of microalgae , 2015 .

[12]  C.P.M. Vimalabai,et al.  Effects of Prolonged UV-B Enhanced Fluorescent Radiation on Some Marine Microalgae , 2002, Biologia Plantarum.

[13]  Kae Sato,et al.  Power-free poly(dimethylsiloxane) microfluidic devices for gold nanoparticle-based DNA analysis. , 2004, Lab on a chip.