The epidemiological characteristics and genetic diversity of dengue virus during the third largest historical outbreak of dengue in Guangdong, China, in 2014.

OBJECTIVES The third largest historical outbreak of dengue occurred during July to December 2014, in 20 of 21 cities of Guangdong, China. The epidemiological and molecular characteristics of the introduction, expansion and phylogeny of the DENV isolates involved in this outbreak were investigated. METHODS A combination analyses of epidemiological characteristics and genetic diversity of dengue virus was performed in this study. RESULTS In total, 45,236 cases and 6 fatalities were reported. Unemployed individuals, retirees and retailers were the most affected populations. A total of 6024 cases were verified to have DENV infections by nucleic acid detection, of which 5947, 74 and 3 were confirmed to have DENV-1, -2, and -3 infections, respectively. Phylogenetic analyses of DENV-1 isolates were assigned into three genotypes (I, IV, and V). Genotype V was the predominant genotype that likely originated from Singapore. The DENV-2 isolates were assigned to the Cosmopolitan and Asian I genotypes. A unique DENV-3 isolate (genotype III) shared high similarity with isolates obtained from Guangdong in 2013. CONCLUSIONS A combination analyses demonstrated the multiple geographical origins of this outbreak, and highlight the importance of early detection, the case management and vector surveillance for preventing further dengue epidemics in Guangdong.

[1]  Lei Luo,et al.  Emergence of dengue virus 4 genotype II in Guangzhou, China, 2010: Survey and molecular epidemiology of one community outbreak , 2012, BMC Infectious Diseases.

[2]  E. Massad,et al.  The cost of dengue control , 2011, The Lancet.

[3]  Shuiping Chen.  The origin of dengue viruses caused the DF outbreak in Guangdong province, China, in 2006. , 2011, Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases.

[4]  Xiaobo Liu,et al.  Dengue is still an imported disease in China: a case study in Guangzhou. , 2015, Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases.

[5]  A. James,et al.  Dengue Fever in mainland China. , 2010, The American journal of tropical medicine and hygiene.

[6]  G. Chang,et al.  Development of Real-Time Reverse Transcriptase PCR Assays To Detect and Serotype Dengue Viruses , 2006, Journal of Clinical Microbiology.

[7]  Xiao-Guang Chen,et al.  A local outbreak of dengue caused by an imported case in Dongguan China , 2012, BMC Public Health.

[8]  Guohua Chen,et al.  An outbreak of dengue virus serotype 1 infection in Cixi, Ningbo, People's Republic of China, 2004, associated with a traveler from Thailand and high density of Aedes albopictus. , 2007, The American journal of tropical medicine and hygiene.

[9]  A Heddini,et al.  Increased number of dengue cases in Swedish travellers to Thailand. , 2009, Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin.

[10]  Isolation, identification and computational studies on Pseudomonas aeruginosa sp. strain MPC1 in tannery effluent , 2011, Bioinformation.

[11]  Zheng Neng.  ANALYSIS ON THE RESULT OF EPIDEMIOLOGICAL DETECTION OF DENGUE IN FUZHOU CITY FROM 1999 TO 2001. , 2002 .

[12]  T. Monath,et al.  Dengue: the risk to developed and developing countries. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[13]  L. Luo,et al.  Molecular characterization of the envelope gene of dengue virus type 3 newly isolated in Guangzhou, China, during 2009-2010. , 2013, International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases.

[14]  T. Scott,et al.  Consequences of the Expanding Global Distribution of Aedes albopictus for Dengue Virus Transmission , 2010, PLoS neglected tropical diseases.

[15]  S. Usuku,et al.  Phylogenetic analysis of dengue-3 viruses prevalent in Guatemala during 1996–1998 , 2001, Archives of Virology.

[16]  L. Luo,et al.  Re-Emergence of Dengue Virus Type 3 in Canton, China, 2009–2010, Associated with Multiple Introductions through Different Geographical Routes , 2013, PloS one.

[17]  C. Sousa,et al.  Ongoing outbreak of dengue type 1 in the Autonomous Region of Madeira, Portugal: preliminary report. , 2012, Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin.

[18]  D. Gubler,et al.  Dengue and dengue haemorrhagic fever , 1998, The Lancet.

[19]  D. Gubler,et al.  Dengue in China: a clinical review. , 1993, Bulletin of the World Health Organization.

[20]  David L. Smith,et al.  Global temperature constraints on Aedes aegypti and Ae. albopictus persistence and competence for dengue virus transmission , 2014, Parasites & Vectors.

[21]  N. Mühlberger,et al.  Epidemiology and clinical features of imported dengue fever in Europe: sentinel surveillance data from TropNetEurop. , 2002, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[22]  Jian Wang,et al.  Co-circulation of two genotypes of dengue virus serotype 3 in Guangzhou, China, 2009 , 2012, Virology Journal.

[23]  W. J. McBride,et al.  Dengue viral infections; pathogenesis and epidemiology. , 2000, Microbes and infection.

[24]  Jian Wang,et al.  Severe dengue outbreak in Yunnan, China, 2013. , 2014, International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases.

[25]  D. Gubler,et al.  Emergence and Global Spread of a Dengue Serotype 3, Subtype III Virus , 2003, Emerging infectious diseases.

[26]  T. Cosgriff,et al.  The reemergence of dengue in China. , 1989, Reviews of infectious diseases.

[27]  L. Luo,et al.  Molecular epidemiological and virological study of dengue virus infections in Guangzhou, China, during 2001–2010 , 2013, Virology Journal.

[28]  G. Malavige,et al.  Dengue viral infections , 2004, Postgraduate Medical Journal.

[29]  E. Holmes,et al.  Extinction and rapid emergence of strains of dengue 3 virus during an interepidemic period. , 2002, Virology.

[30]  Weijun Chen,et al.  Molecular epidemiology of dengue viruses in southern China from 1978 to 2006 , 2011, Virology Journal.

[31]  Qiyong Liu,et al.  Dengue Virus Serotype 3 Subtype III, Zhejiang Province, China , 2011, Emerging infectious diseases.

[32]  Yan Sun,et al.  Evolutionary history and spatiotemporal dynamics of dengue virus type 1 in Asia. , 2013, Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases.